LOCUS       sequence-83035-e       10441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Erwinia herbicola Eho10 genes crtE and crtB, together with 
            the pds (crtP) gene of Synechococcus PCC7942 fused to the lacZ gene 
            at the N terminus. Produces zeta-carotene in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Cunningham FX Jr, Sun Z, Chamovitz D, Hirschberg J, Gantt E
  TITLE     Molecular structure and enzymatic function of lycopene cyclase from 
            the cyanobacterium Synechococcus sp strain PCC7942.
  JOURNAL   Plant Cell. 1994 Aug;6(8):1107-21.
  PUBMED    7919981
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Cell."; date: "1994-08"; volume: "6(8)"; pages: "1107-21"
COMMENT     Sequence Label: pAC-ZETA
FEATURES             Location/Qualifiers
     source          1..10441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1505..1510,1511..1527,1528..1533)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(5794..5813)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    6063..6084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6099..6104,6105..6122,6123..6129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6137..6153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6142..6164
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6161..6177
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6161..6177
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6196..6216
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6198..6216
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      7446..7455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(10001..10441,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
     1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
     1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
     1861 ccgtcctgtg gatctaaagg cacagcgtct catgcttcgc acaatgtaaa actgcttcag
     1921 aacctggcga gagctatccg cgcggtctac ggttaactga tactaaaaga caattcagcg
     1981 ggtaaccttg caatggtgag tggcagtaaa gcgggcgttt cgcctcatcg cgaaatagaa
     2041 gtaatgagac aatccattga cgatcacctg gctggcctgt tacctgaaac cgacagccag
     2101 gatatcgtca gccttgcgat gcgtgaaggc gtcatggcac ccggtaaacg gatccgtccg
     2161 ctgctgatgc tgctggccgc ccgcgacctc cgctaccagg gcagtatgcc tacgctgctc
     2221 gatctcgcct gcgccgttga actgacccat accgcgtcgc tgatgctcga cgacatgccc
     2281 tgcatggaca acgccgagct gcgccgcggt cagcccacta cccacaaaaa atttggtgag
     2341 agcgtggcga tccttgcctc cgttgggctg ctctctaaag cctttggtct gatcgccgcc
     2401 accggcgatc tgccggggga gaggcgtgcc caggcggtca acgagctctc taccgccgtg
     2461 ggcgtgcagg gcctggtact ggggcagttt cgcgatctta acgatgccgc cctcgaccgt
     2521 acccctgacg ctatcctcag caccaaccac ctcaagaccg gcattctgtt cagcgcgatg
     2581 ctgcagatcg tcgccattgc ttccgcctcg tcgccgagca cgcgagagac gctgcacgcc
     2641 ttcgccctcg acttcggcca ggcgtttcaa ctgctggacg atctgcgtga cgatcacccg
     2701 gaaaccggta aagatcgcaa taaggacgcg ggaaaatcga cgctggtcaa ccggctgggc
     2761 gcagacgcgg cccggcaaaa gctgcgcgag catattgatt ccgccgacaa acacctcact
     2821 tttgcctgtc cgcagggcgg cgccatccga cagtttatgc atctgtggtt tggccatcac
     2881 cttgccgact ggtcaccggt catgaaaatc gcctgatacc gcccttttgg gttcaagcag
     2941 tacataacga tggaaccaca ttacaggagt agtgatgaat gaaggacgag cgccttgttc
     3001 agcgtaagaa cgatcatctg gatatcgttc tcgacccccg tcgcgccgta actcaggcta
     3061 gcgcaggttt tgagcgctgg cgctttaccc actgcgccct gccagagctg aattttagcg
     3121 acatcacgct ggaaaccacc ttcctgaatc ggcagctaca ggctccgctg ctgatcagct
     3181 ccatgaccgg cggcgttgag cgctcgcgcc atatcaaccg ccacctcgcc gaggcggcgc
     3241 aggtgctaaa aattgcgatg ggggtgggct cccagcgcgt cgccattgag agcgacgcgg
     3301 gcttagggct ggataaaacc ctgcggcagc tggctccgga cgtgccgctg ctggcgaacc
     3361 tcggcgcggc gcagctgacc ggcagaaaag gtattgatta cgcccgacgg gccgtggaga
     3421 tgatcgaggc ggatgcgctg attgtgcacc taaacccgct gcaggaggcg ctacagcccg
     3481 gcggcgatcg cgactggcgc ggacggctgg cggctattga aactctggtc cgcgagctgc
     3541 ccgttccgct ggtggtgaaa gaggtgggag ccggtatctc ccgaaccgtg gccgggcagc
     3601 tgatcgatgc cggcgttacc gtgattgacg tcgcgggcgc gggcggcacc agctgggccg
     3661 ccgttgaagg cgagcgggcg gccaccgagc agcagcgcag cgtggccaac gtctttgccg
     3721 actgggggat ccccaccgct gaggcgctgg ttgacattgc cgaggcctgg ccgcagatgc
     3781 cccttattgc ctcgggcggg attaaaaacg gcgtcgagat ctttaccggc agcgcgctgg
     3841 cggatgactt ctcgctctac ctgcactcgc cctgcgtgac cgatccctcg ctcgcgcctc
     3901 ccggctgcgc cagcttctac gtgctggccc cggtgccgca tcttggcaac gcgccgctgg
     3961 actgggcgca ggaggggccg aagctgcgcg accgcatctt tgactacctt gaagagcgct
     4021 atatgcccgg cctgcgtagc cagctggtga cccagcggat ctttaccccg gcagacttcc
     4081 acgacacgct ggatgcgcat ctgggatcgg ccttctccat cgagccgctg ctgacccaaa
     4141 gcgcctggtt ccgcccgcac aaccgcgaca gcgacattgc caacctctac ctggtgggcg
     4201 caggtactca ccctggggcg ggcattcctg gcgtagtggc ctcggcgaaa gccaccgcca
     4261 gcctgatgat tgaggatctg caatgagcca accgccgctg cttgaccacg ccacgcagac
     4321 catggccaac ggctcgaaaa gttttgccac cgctgcgaag ctgttcgacc cggccacccg
     4381 ccgtagcgtg ctgatgctct acacctggtg ccgccactgc gatgacgtca ttgacgacca
     4441 gacccacggc ttcgccagcg aggccgcggc ggaggaggag gccacccagc gcctggcccg
     4501 gctgcgcacg ctgaccctgg cggcgtttga aggggccgag atgcaggatc cggccttcgc
     4561 tgcctttcag gaggtggcgc tgacccacgg tattacgccc cgcatggcgc tcgatcacct
     4621 cgacggcttt gcgatggacg tggctcagac ccgctatgtc acctttgagg atacgctgcg
     4681 ctactgctat cacgtggcgg gcgtggtggg tctgatgatg gccagggtga tgggcgtgcg
     4741 ggatgagcgg gtgctggatc gcgcctgcga tctggggctg gccttccagc tgacgaatat
     4801 cgcccgggat attattgacg atgcggctat tgaccgctgc tatctgcccg ccgagtggct
     4861 gcaggatgcc gggctgaccc cggagaacta tgccgcgcgg gagaatcggg ccgcgctggc
     4921 gcgggtggcg gagcggctta ttgatgccgc agagccgtac tacatctcct cccaggccgg
     4981 gctacacgat ctgccgccgc gctgcgcctg ggcgatcgcc accgcccgca gcgtctaccg
     5041 ggagatcggt attaaggtaa aagcggcggg aggcagcgcc tgggatcgcc gccagcacac
     5101 cagcaaaggt gaaaaaattg ccatgctgat ggcggcaccg gggcaggtta ttcgggcgaa
     5161 gacgacgagg gtgacgccgc gtccggccgg tctttggcag cgtcccgttt aggcgggcgg
     5221 ccatgacgtt cacgcaggat cgcctgtagg tcggcaggct tgcgggcgta aataaaaccg
     5281 aaggagacgc agccctcccg gccgcgcacc gcgtggtgca ggcggtgggc gacgtagagc
     5341 cgcttcaggt agccccggcg cgggatcccg cctcgccgtg ctgtccggtc tcaacctgat
     5401 ccgccagaat cgagccaacg ggatcggcca gcacgaattc ggtatgcgga gagtgtttgg
     5461 caaaccatgc ctgcaagcca cccagcgtgc cgccggagcc aacgcctact accacggcat
     5521 caaccctgcc tgccagctgg tcgaacagct ccggtgcggt ggtggtggcg tgcgccagcg
     5581 ggttggccgg gttagagaac tgatcaatat agtaagcacc cggcgtctcc tctgccaggc
     5641 ggcgggcgta gtcctgatag tactccgggt ggccctttgt cacgtcggag cgggtcaggc
     5701 gcacatcaac acccagcgca cgcaggtggt agatcctcta cgccggacgc atcgtggccg
     5761 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     5821 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     5881 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     5941 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     6001 gagagcgtcg actggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     6061 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     6121 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     6181 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctggg tacgcgatcg
     6241 cactttgatc agtgttgaca gcgcgatcgc gaatcagaat tgttacagaa tgcaacgtat
     6301 actaagagac gattcttctg cccttccagc caaaagccat gcgcgtagcg atcgccggtg
     6361 ccggacttgc cggactctcc tgtgccaagt acttggccga tgccggtcat acgcccatcg
     6421 tctatgaacg tcgggacgtc cttggcggca aggttgccgc ttggaaagat gaagacggcg
     6481 actggtacga aactggccta catatctttt ttggggctta ccccaacatg ttgcagctct
     6541 ttaaggagct gaacattgaa gatcgcctgc agtggaagtc ccactcgatg atcttcaacc
     6601 aacccacaaa gccgggcacc tattcgcgct tcgacttccc agacattcca gcgccaatca
     6661 acggtgttgc agcaatcctc agcaacaacg acatgttgac ctgggaagaa aaaatcaagt
     6721 ttggcttggg cttgttgcca gcgatgattc gcggccagtc ctacgtcgaa gagatggatc
     6781 aatactcatg gacggagtgg ctgcgcaaac aaaatattcc agagcgggtc aacgatgaag
     6841 tcttcatcgc catggctaaa gcgctcaact ttattgaccc ggacgaaatt tccgccacgg
     6901 tcgtcctaac ggcactcaac cgcttcttgc aagagaagaa aggttcaatg atggcctttt
     6961 tggatggtgc gccgcccgag cgtctttgcc agccgatcgt cgaacatgtc caagctcgcg
     7021 gtggtgatgt gctgctgaat gcgcctctga aagagttcgt gctcaatgac gacagtagcg
     7081 tccaagcttt tcggattgct ggcatcaaag gtcaagaaga acaactcatt gaggcagatg
     7141 cctacgtttc ggcactgccg gttgatccgc tcaagctact gttgccggat gcatggaaag
     7201 ccatgcccta cttccagcaa ctcgatggtc tgcagggcgt gccggtcatc aacattcacc
     7261 tctggttcga tcgcaagctg accgatatcg atcacctgct gttctcgcga tcgcccctgc
     7321 tcagtgtcta tgccgacatg agtaacacct gtcgcgagta cgaagatccc gatcgctcaa
     7381 tgctagagct ggtcttcgcc cccgccaaag actggattgg ccgctccgac gaagacatct
     7441 tggctgccac catggccgag attgaaaagc tattcccaca gcatttcagc ggtgagaatc
     7501 cggcacgtct gcgcaaatac aaaattgtca aaacgcccct gtcggtctac aaagccacgc
     7561 cgggccgtca acaatatcgc cccgatcaag ctagcccgat cgctaatttc ttcctgaccg
     7621 gcgactacac catgcagcgc tacctcgcca gtatggaagg ggcggtccta tctggtaagc
     7681 tgacagcgca agccatcatt gctcgccaag atgagttgca acgtcgcagc agcggacgac
     7741 cgctggccgc gagtcaggca tagacactgt tacccatgtt gcagttgcta gctctgaagc
     7801 ttcactggtc accctctcac ccccgactcg caccgtctgt ctgatgctgc agatgatacc
     7861 ggcacggcct tcacgggcgc tcgcctctct gtcggaggcc tacgaggagt gccgacaaat
     7921 taccgctcgc tacgctaaga ccttttacct cgggacgctt ttgatgcccg aggccaagcg
     7981 tcaggccatc tgggccatct acgtctggtg tcgtcgtacc gatgaactag tcgatggccc
     8041 tcaagcagcg cagactaact tcgcgaccct ggatgcttgg gagcggcggt tggaacgact
     8101 gtttgctggg gaacccgaag acgactgcga tgttgccttg gtggatacct tggctcgcta
     8161 tcctctcgat atccagccct tccgcgacat gatcgaaggg cagcggatgg atctgctgca
     8221 aaaccgctat tccacttttg aggatctcta cacctactgc taccgtgtcg ccggtactgt
     8281 gggactgatg tcacagccgg tgatgggcat tgaatcgacc aatagtcgtg ccccttggga
     8341 tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     8401 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     8461 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     8521 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     8581 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     8641 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     8701 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     8761 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     8821 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     8881 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     8941 gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca
     9001 ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc
     9061 cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg
     9121 gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
     9181 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
     9241 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
     9301 tccgtgtttc gtaaagtctg gaaacgcgga agtcccctac gtgctgctga agttgcccgc
     9361 aacagagagt ggaaccaacc ggtgatacca cgatactatg actgagagtc aacgccatga
     9421 gcggcctcat ttcttattct gagttacaac agtccgcacc gctgtccggt agctccttcc
     9481 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     9541 tcgtaggaca ggtgccggca gcgcccaaca gtcccccggc cacggggcct gccaccatac
     9601 ccacgccgaa acaagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
     9661 ggctaccctg tggaacacct acatctgtat taacgaagcg ctaaccgttt ttatcaggct
     9721 ctgggaggca gaataaatga tcatatcgtc aattattacc tccacgggga gagcctgagc
     9781 aaactggcct caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc
     9841 ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg
     9901 gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag
     9961 caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
    10021 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg
    10081 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
    10141 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
    10201 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
    10261 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
    10321 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
    10381 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
    10441 g
//
