LOCUS       sequence-83036-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double-GFP reporter for DNA cleavage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pKF251
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     183..201
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     complement(223..242)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    252..333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     277..296
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(496..517)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     845..864
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gagaaggagg caaacagcgc tgactttgag tggaatgtca ttttgagtga gaggtaatcg
       61 aaagaacctg gtacatcaaa tacccttgga tcgaagtaaa tttaaaactg atcagataag
      121 ttcaatgata tccagtgcag taaaaaaaaa aaatgttttt tttatctact ttccgcaaaa
      181 atgggtttta ttaacttaca tacatactag aattcataca ttgatgagtt tggacaaacc
      241 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
      301 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
      361 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
      421 ggtatggctg attatgatct agaggccgct ttacttgtac agctcgtcca tgccgagagt
      481 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      541 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
      601 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
      661 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
      721 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      781 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      841 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      901 gggcatggcg
//
