LOCUS       sequence-83040-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and puromycin resistance from EF-1a promoter.
            3rd generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiGuide-Puro
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      37..172
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(192..211)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(193..211)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(221..238)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(221..237)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(230..252)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      379..834
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(466..485)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     676..697
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        860..964
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
       61 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      121 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
      181 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      241 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      301 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      361 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      421 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      481 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      541 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
      601 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
      661 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
      721 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
      781 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
      841 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      901 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      961 gagtatgagt attca
//
