LOCUS       sequence-83044-e        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and puromycin resistance from EF-1a promoter.
            3rd generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiGuide-Puro
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     141..160
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa
       61 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
      121 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
      181 tcttggcttt atatatcttg tggaaaggac gaaacaccgg agacggttgt aaatgagcac
      241 acaaaataca catgctaaaa tattatattc tatgaccttt ataaaatcaa ccaaaatctt
      301 ctttttaata actttagtat caataattag aatttttatg ttcctttttg caaactttta
      361 ataaaaatga gcaaaataaa aaaacgctag ttttagtaac tcgcgttgtt ttcttcacct
      421 ttaataatag ctactccacc acttgttcct aagcggtcag ctcctgcttc aatcattttt
      481 tgagcatctt caaatgttct aactccacca gctgctttaa ctaaagcatt gtctttaaca
      541 actgacttca ttagtttaac atcttcaaat gttgcacctg attttgaaaa tcctgttgat
      601 gttttaacaa attctaatcc agcttcaaca gctatttcac aagctttcat gatttcttct
      661 tttgttaata aacaattttc cataatacat ttaacaacat gtgatccagc tgcttttttt
      721 acagctttca tgtcttctaa aactaattca taatttttgt cttttaatgc accaatattt
      781 aataccatat caatttctgt tgcaccatct ttaattgctt cagaaacttc gaatgctttt
      841 gtagctgttg tgcatgcacc tagaggaaaa cctacaacat ttgttattcc tacatttgtg
      901 ccttttaata attctttaca atagcttgtt caatatgaat taacacaaac tgttgcaaaa
      961 tcaaattcaa ttgcttcatc acataattgt ttaatttcag ctttcgtagc atcttgtttt
     1021 aataatgtgt ga
//
