LOCUS       sequence-83045-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and puromycin resistance from EF-1a promoter.
            3rd generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiGuide-Puro
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccttatttta acttgctatt tctagctcta aaacagagac gtacaaaaaa gagcaagaag
       61 ctaaaaaaga tttaaaaatt atttttagcg cagttaatgg aacaggaact aaatttaccc
      121 caaaaatatt acgtgaatca ggatataacg ttattgaggt tgaagagcat gcatttgaag
      181 atgaaacatt taaaaatgtt gtaaatccaa atccagaatt tgatcctgca tgaaaaatac
      241 cgcttgaata tggtattaaa catgatgcag atattattat tatgaatgac ccagatgctg
      301 acagatttgg aatggcaata aaacatgatg gtcattttgt aagattagat ggaaatcaaa
      361 caggaccaat tttaattgat tgaaaattat caaatctaaa acgcttaaat agcattccaa
      421 aaaatccggc tctatattca agttttgtaa caagtgattt gggtgataga atcgctcatg
      481 aaaaatatgg agttaatatt gtaaaaactt taactggatt taaatgaatg ggtagagaaa
      541 ttgctaaaga agaagataac ggattaaatt ttgtttttgc ttatgaagaa agttatggat
      601 atgtaattga tgactcagct agagataaag atggaataca agcttctata ttaatagcag
      661 aggctgcttg attttataaa aaacaaaata aaacattagt agactattta gaagatttat
      721 ttaaagaaat gggtgcatat tacactttca ctttaaactt gaattttaaa ccagaagaaa
      781 agaaattaaa aattgaacca ttaatgaaat cattgagagc aacaccctta actcaaattg
      841 ctggacttaa agttgttaat gttgaagact acatcgatgg aatgtataat atgccaggac
      901 aagacttact aaaatttt
//
