LOCUS       sequence-83047-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian bicistronic expression of mouse Olig2 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLP-mOlig2-ires-gfp
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      50..144
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(180..215)
                     /codon_start=1
                     /product="6x(His-Asn) affinity tag"
                     /label=6xHN
                     /translation="HNHNHNHNHNHN"
ORIGIN
        1 actgcgatga gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg actcctgttg
       61 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
      121 cgggcgtttt ttattggtga gaatccaagc actagtcatg accaggatct cctaggctag
      181 ttgtgattat gattatgatt atgattatga ttatgaccac ttacctgggc ccgcgcgcca
      241 aacgaatggt ctagaaagct tgctcctgtg ctctgaaaag gaacattcac agatttgtta
      301 ggaaaccaca aatcaacagg agagacaaca ggagggaagg ccacacttct gagaccacag
      361 gaccctaagt gcttctgata cccacgccag cgtctactaa cttagtgcct ggccctgggc
      421 cagggatgaa cctgcagggt gaggcccaga ggtcgcttct gcagtgcggt tcctgggagt
      481 ctcctacccc gcctgaagtg ggggaaagaa gtcaagtgca gtgctctgcg tctcgtctaa
      541 gcccagacag ggagtcaatc tttgtacacc ggaaagttag tttttagact tcctgggggt
      601 gcaggcagga agttccaggg atgaaagtac ccgcgcgttg tggccgtttt gtacaatggc
      661 gcctcccaga cccttggagt gttcagccaa agagtcaacc aggttctgga acgaacgcat
      721 ctctccatcg cttcaaatgc tcccattgct tcggttccca tatattctac tccccataat
      781 ccccta
//
