LOCUS       sequence-83054-e        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mitochondrial targeted DsRed1-E5 as reporter for 
            mitochondrial content, structure, oxidative stress and damage in 
            vitro and in vivo .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Laker RC, Xu P, Ryall KA, Sujkowski A, Kenwood BM, Chain KH, Zhang 
            M, Royal MA, Hoehn KL, Dirscoll M, Adler PN, Wessells RJ, Saucerman 
            JJ, Yan Z
  TITLE     A novel MitoTimer reporter gene for mitochondrial content, 
            structure, stress and damage in vivo.
  JOURNAL   J Biol Chem. 2014 Apr 25;289(17):12005-12015. doi: 
            10.1074/jbc.M113.530527
  PUBMED    24644293
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1074/jbc.M113"; 
            journalName: "J Biol Chem"; date: "2014-04-25- 25"; volume: "289"; 
            issue: "17"; pages: "12005-12015"
COMMENT     Sequence Label: pMitoTimer
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..119
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      147..156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(153..155,156..158,159..833)
                     /codon_start=1
                     /product="green-to-red fluorescent timer derivative of 
                     DsRed (Terskikh et al., 2000)"
                     /label=Timer
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(336..356)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     748..771
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(991..1010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcatgtcc gtcctgacgc
       61 cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg cgcgccaaga
      121 tccattcgtt gggggatcca ccggtcgcca ccatggtgcg ctcctccaag aacgtcatca
      181 aggagttcat gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac gagttcgaga
      241 tcgagggcga gggcgagggc cgcccctacg agggccacaa caccgtgaag ctgaaggtga
      301 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct
      361 ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg
      421 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggcg accgtgaccc
      481 aggactcctc cctgcaggac ggctgcttca tctacaaggt gaagttcatc ggcgtgaact
      541 tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc tccaccgagc
      601 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca caaggccctg aagctgaagg
      661 acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc
      721 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
      781 ccatcgtgga gcagtacgag cgcaccgagg gccgccacca cctgttcctg tagcggccgc
      841 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
      901 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
      961 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1021 catttttttc actg
//
