LOCUS       sequence-83059-e        7228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid contains POU2F1 (Oct-1) gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Tanaka M, Herr W
  TITLE     Differential transcriptional activation by Oct-1 and Oct-2: 
            interdependent activation domains induce Oct-2 phosphorylation.
  JOURNAL   Cell. 1990 Feb 9;60(3):375-86.
  PUBMED    2302733
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1990-02-9"; volume: "60(3)"; pages: "375-86"
COMMENT     Sequence Label: pCG-Oct-1
FEATURES             Location/Qualifiers
     source          1..7228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     458..478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     504..528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     intron          2917..3489
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3621..3640)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3686..3741
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3740..3759)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        complement(4078..4274)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4092..4227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4341..4358)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4512..5100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4592..4611)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5271..6062,6063..6131))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5894..5913
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6132..6236)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6304..6322
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6360..6382)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6482..6501
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6568..7023)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6705..6726)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6917..6936
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7153..7175
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7167..7184
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7168..7184
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7191..7210
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7191..7209
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(7228,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt
      661 ctagaatgaa caatccgtca gaaaccagta aaccatctat ggagagtgga gatggcaaca
      721 caggcacaca aaccaatggt ctggactttc agaagcagcc tgtgcctgta ggaggagcaa
      781 tctcaacagc ccaggcgcag gctttccttg gacatctcca tcaggtccaa ctcgctggaa
      841 caagtttaca ggctgctgct cagtctttaa atgtacagtc taaatctaat gaagaatcgg
      901 gggattcgca gcagccaagc cagccttccc agcagccttc agtgcaggca gccattcccc
      961 agacccagct tatgctagct ggaggacaga taactgggct tactttgacg cctgcccagc
     1021 aacagttact actccagcag gcacaggcac aggcacagct gctggctgct gcagtgcagc
     1081 agcactccgc cagccagcag cacagtgctg ctggagccac catctccgcc tctgctgcca
     1141 cgcccatgac gcagatcccc ctgtctcagc ccatacagat cgcacaggat cttcaacaac
     1201 tgcaacagct tcaacagcag aatctcaacc tgcaacagtt tgtgttggtg catccaacca
     1261 ccaatttgca gccagcgcag tttatcatct cacagacgcc ccagggccag cagggtctcc
     1321 tgcaagcgca aaatcttcta acgcaactac ctcagcaaag ccaagccaac ctcctacagt
     1381 cgcagccaag catcaccctc acctcccagc cagcaacccc aacacgcaca atagcagcaa
     1441 ccccaattca gacacttcca cagagccagt caacaccaaa gcgaattgat actcccagct
     1501 tggaggagcc cagtgacctt gaggagcttg agcagtttgc caagaccttc aaacaaagac
     1561 gaatcaaact tggattcact cagggtgatg ttgggctcgc tatggggaaa ctatatggaa
     1621 atgacttcag ccaaactacc atctctcgat ttgaagcctt gaacctcagc tttaagaaca
     1681 tgtgcaagtt gaagccactt ttagagaagt ggctaaatga tgcagagaac ctctcatctg
     1741 attcgtccct ctccagccca agtgccctga attctccagg aattgagggc ttgagccgta
     1801 ggaggaagaa acgcaccagc atagagacca acatccgtgt ggccttagag aagagtttct
     1861 tggagaatca aaagcctacc tcggaagaga tcactatgat tgctgatcag ctcaatatgg
     1921 aaaaagaggt gattcgtgtt tggttctgta accgccgcca gaaagaaaaa agaatcaacc
     1981 caccaagcag tggtgggacc agcagctcac ctattaaagc aattttcccc agcccaactt
     2041 cactggtggc gaccacacca agccttgtga ctagcagtgc agcaactacc ctcacagtca
     2101 gccctgtcct ccctctgacc agtgctgctg tgacgaatct ttcagttaca ggcacttcag
     2161 acaccacctc caacaacaca gcaaccgtga tttccacagc gcctccagct tcctcagcag
     2221 tcacgtcccc ctctctgagt ccctcccctt ctgcctcagc ctccacctcc gaggcatcca
     2281 gtgccagtga gaccagcaca acacagacca cctccactcc tttgtcctcc cctcttggga
     2341 ccagccaggt gatggtgaca gcatcaggtt tgcaaacagc agcagctgct gcccttcaag
     2401 gagctgcaca gttgccagca aatgccagtc ttgctgccat ggcagctgct gcaggactaa
     2461 acccaagcct gatggcaccc tcacagtttg cggctggagg tgccttactc agtctgaatc
     2521 cagggaccct gagcggtgct ctcagcccag ctctaatgag caacagtaca ctggcaacta
     2581 ttcaagctct tgcttctggt ggctctcttc caataacatc acttgatgca actgggaacc
     2641 tggtatttgc caatgcggga ggagccccca acatcgtgac tgcccctctg ttcctgaacc
     2701 ctcagaacct ctctctgctc accagcaacc ctgttagctt ggtctctgcc gccgcagcat
     2761 ctgcagggaa ctctgcacct gtagccagcc ttcacgccac ctccacctct gctgagtcca
     2821 tccagaactc tctcttcaca gtggcctctg ccagcggggc tgcgtccacc accaccaccg
     2881 cctccaaggc acagtgagga tcctgagaac ttcagggtga gtttggggac ccttgattgt
     2941 tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg
     3001 ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc
     3061 tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta
     3121 actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat
     3181 ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta
     3241 tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata
     3301 tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacacc
     3361 ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg
     3421 ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttctct
     3481 ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa
     3541 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     3601 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     3661 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     3721 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     3781 cagaatgagt atttggttta gagtttggca acatatgcca tatgctggct gccatgaaca
     3841 aaggtggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     3901 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     3961 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     4021 ctgactactc ccagtcatag ctgtccctct tctcttatga agatcctcga gaagctgttt
     4081 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     4141 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     4201 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     4261 ctaattgaga tgcagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     4321 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     4381 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4441 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4501 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4561 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4621 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4681 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4741 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4801 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4861 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4921 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     4981 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5041 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5101 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5161 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     5221 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5281 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     5341 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     5401 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5461 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5521 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5581 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5641 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5701 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5761 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5821 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5881 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5941 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6001 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6061 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6121 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6181 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6241 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     6301 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     6361 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     6421 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     6481 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     6541 tgcgtaagga gaaaataccg catcaggaaa ttgtaaacgt taatattttg ttaaaattcg
     6601 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     6661 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     6721 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     6781 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     6841 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     6901 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     6961 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     7021 cgtcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     7081 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     7141 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
     7201 cactataggg cgaattcgag ctcgcccc
//
