LOCUS       sequence-83061-e        7276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid contains POU2F1 (Oct-1) gene with HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Tanaka M, Herr W
  TITLE     Differential transcriptional activation by Oct-1 and Oct-2: 
            interdependent activation domains induce Oct-2 phosphorylation.
  JOURNAL   Cell. 1990 Feb 9;60(3):375-86.
  PUBMED    2302733
REFERENCE   2  (bases 1 to 7276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1990-02-9"; volume: "60(3)"; pages: "375-86"
COMMENT     Sequence Label: pCGN-Oct-1
FEATURES             Location/Qualifiers
     source          1..7276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     458..478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     504..528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             666..692
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             672..698
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          2965..3537
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3669..3688)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3734..3789
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3788..3807)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        complement(4126..4322)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4140..4275
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4389..4406)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4560..5148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4640..4659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5319..6110,6111..6179))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5942..5961
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6180..6284)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6352..6370
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6408..6430)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6530..6549
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6616..7071)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6753..6774)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6965..6984
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7201..7223
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7215..7232
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7216..7232
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7239..7258
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7239..7257
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(7276,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt
      661 ctagctatcc ttatgacgtg cctgactatg ccagcctggg aggaccttct agaatgaaca
      721 atccgtcaga aaccagtaaa ccatctatgg agagtggaga tggcaacaca ggcacacaaa
      781 ccaatggtct ggactttcag aagcagcctg tgcctgtagg aggagcaatc tcaacagccc
      841 aggcgcaggc tttccttgga catctccatc aggtccaact cgctggaaca agtttacagg
      901 ctgctgctca gtctttaaat gtacagtcta aatctaatga agaatcgggg gattcgcagc
      961 agccaagcca gccttcccag cagccttcag tgcaggcagc cattccccag acccagctta
     1021 tgctagctgg aggacagata actgggctta ctttgacgcc tgcccagcaa cagttactac
     1081 tccagcaggc acaggcacag gcacagctgc tggctgctgc agtgcagcag cactccgcca
     1141 gccagcagca cagtgctgct ggagccacca tctccgcctc tgctgccacg cccatgacgc
     1201 agatccccct gtctcagccc atacagatcg cacaggatct tcaacaactg caacagcttc
     1261 aacagcagaa tctcaacctg caacagtttg tgttggtgca tccaaccacc aatttgcagc
     1321 cagcgcagtt tatcatctca cagacgcccc agggccagca gggtctcctg caagcgcaaa
     1381 atcttctaac gcaactacct cagcaaagcc aagccaacct cctacagtcg cagccaagca
     1441 tcaccctcac ctcccagcca gcaaccccaa cacgcacaat agcagcaacc ccaattcaga
     1501 cacttccaca gagccagtca acaccaaagc gaattgatac tcccagcttg gaggagccca
     1561 gtgaccttga ggagcttgag cagtttgcca agaccttcaa acaaagacga atcaaacttg
     1621 gattcactca gggtgatgtt gggctcgcta tggggaaact atatggaaat gacttcagcc
     1681 aaactaccat ctctcgattt gaagccttga acctcagctt taagaacatg tgcaagttga
     1741 agccactttt agagaagtgg ctaaatgatg cagagaacct ctcatctgat tcgtccctct
     1801 ccagcccaag tgccctgaat tctccaggaa ttgagggctt gagccgtagg aggaagaaac
     1861 gcaccagcat agagaccaac atccgtgtgg ccttagagaa gagtttcttg gagaatcaaa
     1921 agcctacctc ggaagagatc actatgattg ctgatcagct caatatggaa aaagaggtga
     1981 ttcgtgtttg gttctgtaac cgccgccaga aagaaaaaag aatcaaccca ccaagcagtg
     2041 gtgggaccag cagctcacct attaaagcaa ttttccccag cccaacttca ctggtggcga
     2101 ccacaccaag ccttgtgact agcagtgcag caactaccct cacagtcagc cctgtcctcc
     2161 ctctgaccag tgctgctgtg acgaatcttt cagttacagg cacttcagac accacctcca
     2221 acaacacagc aaccgtgatt tccacagcgc ctccagcttc ctcagcagtc acgtccccct
     2281 ctctgagtcc ctccccttct gcctcagcct ccacctccga ggcatccagt gccagtgaga
     2341 ccagcacaac acagaccacc tccactcctt tgtcctcccc tcttgggacc agccaggtga
     2401 tggtgacagc atcaggtttg caaacagcag cagctgctgc ccttcaagga gctgcacagt
     2461 tgccagcaaa tgccagtctt gctgccatgg cagctgctgc aggactaaac ccaagcctga
     2521 tggcaccctc acagtttgcg gctggaggtg ccttactcag tctgaatcca gggaccctga
     2581 gcggtgctct cagcccagct ctaatgagca acagtacact ggcaactatt caagctcttg
     2641 cttctggtgg ctctcttcca ataacatcac ttgatgcaac tgggaacctg gtatttgcca
     2701 atgcgggagg agcccccaac atcgtgactg cccctctgtt cctgaaccct cagaacctct
     2761 ctctgctcac cagcaaccct gttagcttgg tctctgccgc cgcagcatct gcagggaact
     2821 ctgcacctgt agccagcctt cacgccacct ccacctctgc tgagtccatc cagaactctc
     2881 tcttcacagt ggcctctgcc agcggggctg cgtccaccac caccaccgcc tccaaggcac
     2941 agtgaggatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc
     3001 gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg
     3061 ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta
     3121 ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta
     3181 aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt
     3241 aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca
     3301 gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata
     3361 aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacaccct ggtcatcatc
     3421 ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct
     3481 gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttctcttt cctacagctc
     3541 ctgggcaacg tgctggttgt tgtgctgtct catcattttg gcaaagaatt cactcctcag
     3601 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
     3661 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     3721 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     3781 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     3841 ttggtttaga gtttggcaac atatgccata tgctggctgc catgaacaaa ggtggctata
     3901 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     3961 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     4021 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     4081 agtcatagct gtccctcttc tcttatgaag atcctcgaga agctgtttgc aaaagcctag
     4141 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     4201 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     4261 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     4321 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     4381 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4441 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4501 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4561 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4621 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4681 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4741 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4801 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4861 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4921 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4981 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5041 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5101 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5161 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5221 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5281 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5341 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     5401 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5461 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5521 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5581 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5641 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5701 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5761 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5821 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5881 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5941 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6001 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6061 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6121 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6181 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6241 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6301 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     6361 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     6421 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     6481 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     6541 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     6601 aaataccgca tcaggaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
     6661 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     6721 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     6781 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     6841 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     6901 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     6961 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     7021 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcgcgccatt
     7081 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     7141 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     7201 cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg
     7261 aattcgagct cgcccc
//
