LOCUS       sequence-83081-e        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  serves as PCR-template to generate HR donors for C-terminal YFP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pRB20
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     285..304
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        285..303
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(338..340,341..343,344..856)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(383..404)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(644..663)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     991..1012
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttatttttgc caaataattt ttaacaaaag ctctgaagtc ttcttcattt aaattcttag
       61 atgatacttc atctggaaaa ttgtcccaat tagtagcatc acgctgtgag taagttctaa
      121 accatttttt tattgttgta ttatctctaa tcttactact cgatgagttt tcggtattat
      181 ctctattttt aacttggagc aggttccatt cattgttttt ttcatcatag tgaataaaat
      241 caactgcttt aacacttgtg cctgaacacc atatccatcc ggcgtaatac gactcactat
      301 agggagagcg gccgccagat cttccggatg gctcgagatg gtgagcaagg gcgaggagct
      361 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      421 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      481 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
      541 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      601 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      661 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      721 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      781 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
      841 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      901 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
      961 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1021 cgggatcact
//
