LOCUS       sequence-83093-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian bicistronic expression of mouse Oct3/4 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLP-mOct4-ires-gfp
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      53..146
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(182..217)
                     /codon_start=1
                     /product="6x(His-Asn) affinity tag"
                     /label=6xHN
                     /translation="HNHNHNHNHNHN"
ORIGIN
        1 tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gcactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt gagaatccaa gcactagtca tgaccaggat ctcctaggct
      181 agttgtgatt atgattatga ttatgattat gattatgacc acttacctgg gcccgcgcgc
      241 caaacgaatg gtctagaaag cttctcgagg ggtttgaatg catggagagc ccagagcagt
      301 gacgggaaca gagggaaagg cctcgccctc aggaaaaggg actgagtaga gtgtggtgaa
      361 gtgggggctt ccatagcctg gggtgccaaa gtggggacct gggggcagag gaaaggatac
      421 agccccccct gggaaaggtg tccctgtagc ctcatactct tctcgttggg aatactcaat
      481 acttgatctt ttgcccttct ggcgccggtt acagaaccat actcgaacca catccttctc
      541 tagcccaagc tgattggcga tgtgagtgat ctgctgtagg gagggcttcg ggcacttcag
      601 aaacatggtc tccagactcc acctcacacg gttctcaatg ctagttcgct ttctcttccg
      661 ggcctgcacc agggtctccg atttgcatat ctcctgaagg ttctcattgt tgtcggcttc
      721 ctccacccac ttctccagca ggggccgcag cttacacatg ttcttaaggc tgagctgcaa
      781 ggcctcgaag cgacagatgg tggtctggct gaacaccttt ccaaagagaa cgcccagggt
      841 gagccccacg tcgg
//
