LOCUS       sequence-83095-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral RNAi vector for knockdown of LATS2 and LATS1. 
            Co-expresses EmGFP as reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Yang Z, Nakagawa K, Sarkar A, Maruyama J, Iwasa H, Bao Y, 
            Ishigami-Yuasa M, Ito S, Kagechika H, Hata S, Nishina H, Abe S, 
            Kitagawa M, Hata Y
  TITLE     Screening with a novel cell-based assay for TAZ activators 
            identifies a compound that enhances myogenesis in C2C12 cells and 
            facilitates muscle repair in the muscle injury model.
  JOURNAL   Mol Cell Biol. 2014 Feb 18.
  PUBMED    24550007
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2014 Feb 18."
COMMENT     Sequence Label: pLenti-EmGFP-LATS2/1 KD
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           70..96
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           160..201
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           208..234
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           298..339
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(368..392)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             445..486
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(523..543)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtacaagtaa gctaagcact tcgtggccgt cgatcgttta aagggaggta gtgagtcgac
       61 cagtggatcc tggaggcttg ctgaaggctg tatgctgaat tcatacaggt ccacgttggt
      121 tttggccact gactgaccaa cgtggctgta tgaattcagg acacaaggcc tgttactagc
      181 actcacatgg aacaaatggc ccagatcctg gaggcttgct gaaggctgta tgctgatcac
      241 agtcacattt gaagcggttt tggccactga ctgaccgctt caagtgactg tgatcaggac
      301 acaaggcctg ttactagcac tcacatggaa caaatggccc agatctggcc gcactcgaga
      361 tatctagacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
      421 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
      481 tctacgcgta ccggttagta atgagtttgg aattaattct gtggaatgtg tgtcagttag
      541 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      601 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      661 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
      721 ctccgcccag ttccgcccat tctccgccc
//
