LOCUS       sequence-83101-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the
            linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI 
            /ClaI). Plasmid confers ampicillin resistance to DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: p413GAL1-Zipp.-L-IFP-F[2] (Yeast)
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..199)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     211..229
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta taatgttaca
       61 tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga aatataaata acgttcttaa
      121 tactaacata actataaaaa aataaatagg gacctagact tcaggttgtc taactccttc
      181 cttttcggtt agagcggatg tggggggagg gcgtgaatgt aagcgtgaca taactaatta
      241 catgactcga gttaggcttc tttcctctgc acctgcccgg acagctttct ccccagctcc
      301 tccagggtgg tccgcagatc tggtggcagc acgtagggtg tctgatggtg gcagacaatc
      361 agtccccaca gctggccgcc gaccacgacg ctgacgctca gggaggagcc cactcccata
      421 ttcctcaggt actgcatgtg cattggggat gtagctctga ggacagctcc gcccagaggt
      481 gtaggagcat ttgtctgggg attcagcacg gggtcgaggg gcactgctgc agccctggtg
      541 tctgcggtca gccggagcag gtgtcttgtg tagagggccc tagcttgtgc aggggtgtgg
      601 gatgcgggaa acctgtggcc cagaaaagcc tgcatgccct cccgtctggc ctcagcaatc
      661 atctctccag tggcgtcagg ggcaaatttg tacagcatca cccgatcgaa gccagtcagt
      721 tctctcacag tttgtgttgc gacttcggcc agagcccgca ggtttggagc gctttccaga
      781 gcaaacatgg cgttcctcag ggcgtgaggg ccaatgctgt cccgagctcc ggaggaccca
      841 ccacctccag agccaccgcc accatcgatg cgttcgccaa ctaatttctt taatctggca
      901 acctcatttt
//
