LOCUS       sequence-83102-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of constitutively active Ste7 kinase in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Mok J, Kim PM, Lam HY, Piccirillo S, Zhou X, Jeschke GR, Sheridan 
            DL, Parker SA, Desai V, Jwa M, Cameroni E, Niu H, Good M, Remenyi A,
            Ma JL, Sheu YJ, Sassi HE, Sopko R, Chan CS, De Virgilio C, 
            Hollingsworth NM, Lim WA, Stern DF, Stillman B, Andrews BJ, Gerstein
            MB, Snyder M, Turk BE
  TITLE     Deciphering protein kinase specificity through large-scale analysis 
            of yeast phosphorylation site motifs.
  JOURNAL   Sci Signal. 2010 Feb 16;3(109):ra12. doi: 10.1126/scisignal.2000482.
  PUBMED    20159853
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2010-02-16"; volume: "3(109):ra12. doi"; pages: " 
            10.1126/scisignal.2000482"
COMMENT     Sequence Label: pGEX4T2-Ste7-EE
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctggttccgc gtggatccat ggttcaacga aagactttac agagaaggaa cttgaaaggg
       61 ctcaatctta acctgcaccc agatgtgggc aataatggcc aattgcagga aaagacagag
      121 actcaccagg gacaatctcg aatagaaggc cacgtgatgt ctaacattaa tgcaatacag
      181 aataatagca acctgttttt gcgaagaggc ataaaaaaaa aactgacgtt ggatgcgttt
      241 ggtgatgacc aagctatatc gaaaccaaac actgtggtaa tacagcaacc gcaaaatgaa
      301 cctgttttag ttctgtcttc tctatcacaa tccccgtgtg tatcatcatc atcatctttg
      361 tccacgccat gcattataga tgcgtacagt aataatttcg gattatcgcc atcatccacg
      421 aattctactc cctctacgat tcagggattg tccaatattg caacaccagt tgaaaacgaa
      481 cattcgatat cactaccacc tttggaggaa agcctatcgc cagccgcagc agatctgaaa
      541 gatacgttgt cgggaacttc aaatggtaat tatatacaac tccaggactt ggttcagttg
      601 gggaaaattg gtgctggaaa ttctggaact gtggtgaagg cactacatgt tcctgattcc
      661 aaaatagttg ccaaaaaaac cattcctgtg gaacagaata acagtacaat catcaaccaa
      721 ttagttaggg aattatctat cgtcaaaaac gttaagcccc atgaaaacat tatcaccttc
      781 tatggagctt tattata
//
