LOCUS       sequence-83104-e        2728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  full-length native Gal4 with stop in Gateway middle entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL
  TITLE     Gal80 intersectional regulation of cell-type specific expression in 
            vertebrates.
  JOURNAL   Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 
            2011 Sep 8.
  PUBMED    21905164
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.22734"; journalName: "Dev Dyn"; date: "2011-10"; 
            volume: "240"; issue: "10"; pages: "2324-34"
COMMENT     Sequence Label: pME-Gal4
FEATURES             Location/Qualifiers
     source          1..2728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             70..2715
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
ORIGIN
        1 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
       61 ggctagacca tgaagctact gtcttctatc gaacaagcat gcgatatttg ccgacttaaa
      121 aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa caactgggag
      181 tgtcgctact ctcccaaaac caaaaggtct ccgctgacta gggcacatct gacagaagtg
      241 gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg agaagacctt
      301 gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac aggattattt
      361 gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt ggagactgat
      421 atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga agagagtagt
      481 aacaaaggtc aaagacagtt gactgtatcg attgactcgg cagctcatca tgataactcc
      541 acaattccgt tggattttat gcccagggat gctcttcatg gatttgattg gtctgaagag
      601 gatgacatgt cggatggctt gcccttcctg aaaacggacc ccaacaataa tgggttcttt
      661 ggcgacggtt ctctcttatg tattcttcga tctattggct ttaaaccgga aaattacacg
      721 aactctaacg ttaacaggct cccgaccatg attacggata gatacacgtt ggcttctaga
      781 tccacaacat cccgtttact tcaaagttat ctcaataatt ttcaccccta ctgccctatc
      841 gtgcactcac cgacgctaat gatgttgtat aataaccaga ttgaaatcgc gtcgaaggat
      901 caatggcaaa tcctttttaa ctgcatatta gccattggag cctggtgtat agagggggaa
      961 tctactgata tagatgtttt ttactatcaa aatgctaaat ctcatttgac gagcaaggtc
     1021 ttcgagtcag gttccataat tttggtgaca gccctacatc ttctgtcgcg atatacacag
     1081 tggaggcaga aaacaaatac tagctataat tttcacagct tttccataag aatggccata
     1141 tcattgggct tgaataggga cctcccctcg tccttcagtg atagcagcat tctggaacaa
     1201 agacgccgaa tttggtggtc tgtctactct tgggagatcc aattgtccct gctttatggt
     1261 cgatccatcc agctttctca gaatacaatc tccttccctt cttctgtcga cgatgtgcag
     1321 cgtaccacaa caggtcccac catatatcat ggcatcattg aaacagcaag gctcttacaa
     1381 gttttcacaa aaatctatga actagacaaa acagtaactg cagaaaaaag tcctatatgt
     1441 gcaaaaaaat gcttgatgat ttgtaatgag attgaggagg tttcgagaca ggcaccaaag
     1501 tttttacaaa tggatatttc caccaccgct ctaaccaatt tgttgaagga acacccttgg
     1561 ctatccttta caagattcga actgaagtgg aaacagttgt ctcttatcat ttatgtatta
     1621 agagattttt tcactaattt tacccagaaa aagtcacaac tagaacagga tcaaaatgat
     1681 catcaaagtt atgaagttaa acgatgctcc atcatgttaa gcgatgcagc acaaagaact
     1741 gttatgtctg taagtagcta tatggacaat cataatgtca ccccatattt tgcctggaat
     1801 tgttcttatt acttgttcaa tgcagtccta gtacccataa agactctact ctcaaactca
     1861 aaatcgaatg ctgagaataa cgagaccgca caattattac aacaaattaa cactgttctg
     1921 atgctattaa aaaaactggc cacttttaaa atccagactt gtgaaaaata cattcaagta
     1981 ctggaagagg tatgtgcgcc gtttctgtta tcacagtgtg caatcccatt accgcatatc
     2041 agttataaca atagtaatgg tagcgccatt aaaaatattg tcggttctgc aactatcgcc
     2101 caatacccta ctcttccgga ggaaaatgtc aacaatatca gtgttaaata tgtttctcct
     2161 ggctcagtag ggccttcacc tgtgccattg aaatcaggag caagtttcag tgatctagtc
     2221 aagctgttat ctaaccgtcc accctctcgt aactctccag tgacaatacc aagaagcaca
     2281 ccttcgcatc gctcagtcac gccttttcta gggcaacagc aacagctgca atcattagtg
     2341 ccactgaccc cgtctgcttt gtttggtggc gccaatttta atcaaagtgg gaatattgct
     2401 gatagctcat tgtccttcac tttcactaac agtagcaacg gtccgaacct cataacaact
     2461 caaacaaatt ctcaagcgct ttcacaacca attgcctcct ctaacgttca tgataacttc
     2521 atgaataatg aaatcacggc tagtaaaatt gatgatggta ataattcaaa accactgtca
     2581 cctggttgga cggaccaaac tgcgtataac gcgtttggaa tcactacagg gatgtttaat
     2641 accactacaa tggatgatgt atataactat ctattcgatg atgaagatac cccaccaaac
     2701 ccaaaaaaag agtaagaccc agctttct
//
