LOCUS       sequence-83105-e        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetically encodable fluorescent calcium indicator protein to 
            monitor neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, 
            Hogue IB, Enquist LW, Murthy M, Wang SS
  TITLE     Fast GCaMPs for improved tracking of neuronal activity.
  JOURNAL   Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170.
  PUBMED    23863808
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-07-17"; volume: "4:2170. doi"; pages: " 
            10.1038/ncomms3170"
COMMENT     Sequence Label: Fast-GCaMP-EF09
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(67..510)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTINFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     626..645
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     885..906
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1012..1033)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cttctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgctcac ttcgctgtca tcatttgtac aaactcttcg tagtttacct gaccatcccc
      121 atcgatgtct gcttccctga tcatttcatc aacctcttca tctgttaact tctctccaag
      181 gtttgtcatc acgtggcgaa gctctgctgc actgatgtag ccattgccat ccttatcaaa
      241 cacacggaac gcttctctaa tttcttcttc actgtctgtg tctttcattt ttcttgccat
      301 cattgtcagg aactcaggga agttgattgt gccgtcaccg tcggcatcta cttcattgat
      361 catgtcctgc agctctgctt ctgtggggtt ctgccccaga gaccgcatca ccgtccccag
      421 ctccttggtt gttattgtcc catccccgtc cttgtcaaat agggagaaag cctctttaaa
      481 ttctgcgatc tgctcttcag tcagttggtc acgcgtgttg tactccagct tgtgccccag
      541 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      601 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      661 gcgctcctgg atgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
      721 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
      781 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
      841 atcgccctca ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      901 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgctccctcc
      961 ggtaccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
     1021 cagcaggacc atgt
//
