LOCUS       sequence-83109-e        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetically encodable fluorescent calcium indicator protein to 
            monitor neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, 
            Hogue IB, Enquist LW, Murthy M, Wang SS
  TITLE     Fast GCaMPs for improved tracking of neuronal activity.
  JOURNAL   Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170.
  PUBMED    23863808
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-07-17"; volume: "4:2170. doi"; pages: " 
            10.1038/ncomms3170"
COMMENT     Sequence Label: Fast-GCaMP-EF20
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(69..512)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGNGTINFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     628..647
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     887..908
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1014..1035)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt
       61 gcggccgctc acttcgctgt catcatttgt acaaactctt cgtagtttac ctgaccatcc
      121 ccatcgatgt ctgcttccct gatcatttca tcaacctctt catctgttaa cttctctcca
      181 aggtttgtca tcacgtggcg aagctctgct gcactgatgt agccattgcc atccttatca
      241 aacacacgga acgcttctct aatttcttct tcactgtctg tgtctttcat ttttcttgcc
      301 atcattgtca ggaactcagg gaagttgatt gtgccgttac cgtcggcatc tacttcattg
      361 atcatgtcct gcagctctgc ttctgtgggg ttctgcccca gagaccgcat caccgtcccc
      421 agctccttgg ttgttattgt cccatccccg tccttgtcaa atagggagaa agcctcttta
      481 aattctgcga tctgctcttc agtcagttgg tcacgcgtgt tgtactccag cttgtgcccc
      541 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      601 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      661 gtgcgctcct ggatgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
      721 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc
      781 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
      841 gcatcgccct caccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      901 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catgctccct
      961 ccggtaccgc ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
     1021 tccagcagga ccatgtgatc gcgcttc
//
