LOCUS       sequence-83114-e        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetically encodable fluorescent calcium indicator protein to 
            monitor neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, 
            Hogue IB, Enquist LW, Murthy M, Wang SS
  TITLE     Fast GCaMPs for improved tracking of neuronal activity.
  JOURNAL   Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170.
  PUBMED    23863808
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-07-17"; volume: "4:2170. doi"; pages: " 
            10.1038/ncomms3170"
COMMENT     Sequence Label: Fast-GCaMP-RS08
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..56)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(72..515)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMQNTGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     631..650
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     890..911
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcctttcctt ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg
       61 agtgcggccg ctcacttcgc tgtcatcatt tgtacaaact cttcgtagtt tacctgacca
      121 tccccatcga tgtctgcttc cctgatcatt tcatcaacct cttcatctgt taacttctct
      181 ccagtgttct gcatcacgtg gcgaagctct gctgcactga tgtagccatt gccatcctta
      241 tcaaacacac ggaacgcttc tctaatttct tcttcactgt ctgtgtcttt catttttctt
      301 gccatcattg tcaggaactc agggaagtcg attgtgccgt caccgtcggc atctacttca
      361 ttgatcatgt cctgcagctc tgcttctgtg gggttctgcc ccagagaccg catcaccgtc
      421 cccagctcct tggttgttat tgtcccatcc ccgtccttgt caaataggga gaaagcctct
      481 ttaaattctg cgatctgctc ttcagtcagt tggtcacgcg tgttgtactc cagcttgtgc
      541 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      601 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
      661 atggtgcgct cctggatgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
      721 atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg
      781 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
      841 gtggcatcgc cctcaccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      901 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatgctc
      961 cctccggtac cgcccttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
     1021 aactccagca gga
//
