LOCUS       sequence-83116-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetically encodable fluorescent calcium indicator protein to 
            monitor neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Sun XR, Badura A, Pacheco DA, Lynch LA, Schneider ER, Taylor MP, 
            Hogue IB, Enquist LW, Murthy M, Wang SS
  TITLE     Fast GCaMPs for improved tracking of neuronal activity.
  JOURNAL   Nat Commun. 2013 Jul 17;4:2170. doi: 10.1038/ncomms3170.
  PUBMED    23863808
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-07-17"; volume: "4:2170. doi"; pages: " 
            10.1038/ncomms3170"
COMMENT     Sequence Label: Fast-GCaMP-RS12
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(68..511)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     627..646
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     886..907
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttccctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgctca cttcgctgtc atcatttgta caaactcttc gtagtttacc tgaccatccc
      121 catcgatgtc tgcttccctg atcatttcat caacctcttc atctgttaac ttctctccaa
      181 ggtttgtcat cacgtggcga agctctgctg cactgatgta gccattgcca tccttatcaa
      241 acacacggaa cgcttctcta atttcttctt cactgtctgt gtctttcatt tttcttgcca
      301 tcattgtcag gaactcaggg aagtcgattg tgccgtcacc gtcggcatct acttcattga
      361 tcatgtcctg cagctctgct tctgtggggt tctgccccag agaccgcatc accgtcccca
      421 gctccttggt tgttattgtc ccatccccgt ccttgtcaaa tagggagaaa gcctctttaa
      481 attctgcgat ctgctcttca gtcagttggt cacgcgtgtt gtactccagc ttgtgcccca
      541 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
      601 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
      661 tgcgctcctg gatgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
      721 ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc
      781 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
      841 catcgccctc accctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
      901 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atgctccctc
      961 cggtaccgcc cttgtacagc tcgtccatgc c
//
