LOCUS       sequence-83145-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as 41022, with additional linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  TITLE     various
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-kanMX-tetO-Pcyc1-3xFLAG-LINKER
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..101)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    join(321..339,340..362,363..381,382..404,405..423,424..446,
                     447..465,466..488,489..507,508..530,531..549,550..572,
                     573..591)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ggcgcgccac cagcaccgta atcagcaagt ttatcatcat cgtccttata gtccttatcg
       61 tcgtcatctt tgtaatcctt gtcatcgtcg tccttgtagt ccatattaat taacctccag
      121 gatgataaac ggatcccccg aattgatccg gtaatttagt gtgtgtattt gtgtttgcgt
      181 gtctatagaa gtatagtaat ttatgctaca aaggacctaa tgtataagga aagaatattt
      241 agagaaaaga agaaaacaag agttttatat acatacagag cacatgcatg ccatagggta
      301 ccgagctcga ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact
      361 tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag
      421 ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact
      481 ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac
      541 tgatagggag tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac
      601 tcgatcgagg aattgatcta tattacccag atctgtttag cttgcctcgt ccccgccggg
      661 tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag
      721 ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc
      781 atttgcatcc atacattttg atggccgcac ggcgcgaagc aa
//
