LOCUS       sequence-83149-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Tamai K, Zeng X, Liu C, Zhang X, Harada Y, Chang Z, He X
  TITLE     A mechanism for Wnt coreceptor activation.
  JOURNAL   Mol Cell. 2004 Jan 16. 13(1):149-56.
  PUBMED    14731402
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2004 Jan 16. 13(1):149-56."
COMMENT     Sequence Label: LRP6-pCS2-VSVG
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             210..242
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat ccagagaaga
      121 gaacgcgaga agggaagatg ggggccgtcc tgaggagcct cctggcctgc agcttctgtg
      181 tgctcctgag agcggcccct ttgttgcttt atactgatat cgaaatgaac cgcctgggta
      241 aggctccgga acctttgttg ctttatgcaa acagacggga cttgcgattg gttgatgcta
      301 caaatggcaa agagaatgct acgattgtag ttggaggctt ggaggatgca gctgcggtgg
      361 actttgtgtt tagtcatggc ttgatatact ggagtgatgt cagcgaagaa gccattaaac
      421 gaacagaatt taacaaaact gagagtgtgc agaatgttgt tgtttctgga ttattgtccc
      481 ccgatgggct ggcatgtgat tggcttggag aaaaattgta ctggacagat tctgaaacta
      541 atcggattga agtttctaat ttagatggat ctttacgaaa agttttattt tggcaagagt
      601 tggatcaacc cagagctatt gccttagatc cttcaagtgg gttcatgtac tggacagact
      661 ggggagaagt gccaaagata gaacgtgctg gaatggatgg ttcaagtcgc ttcattataa
      721 taaacagtga aatttactgg ccaaatggac tgactttgga ttatgaagaa caaaagcttt
      781 attgggcaga tgcaaaactt aatttcatcc acaaatcaaa tctggatgga acaaatcggc
      841 aggcagtggt taaaggttcc cttccacatc cttttgcctt gacgttattt gaggacatat
      901 tgtactggac tgactggagc acacactcca ttttggcttg caacaagtat actggtgagg
      961 gtctgcgtg
//
