LOCUS       sequence-83153-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses luciferase under 1.35 kb muscle creatine kinase 
            promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Larochelle N, Lochmuller H, Zhao J, Jani A, Hallauer P, Hastings KE,
            Massie B, Prescott S, Petrof BJ, Karpati G, Nalbantoglu J
  TITLE     Efficient muscle-specific transgene expression after 
            adenovirus-mediated gene transfer in mice using a 1.35 kb muscle 
            creatine kinase promoter/enhancer.
  JOURNAL   Gene Ther. 1997 May;4(5):465-72.
  PUBMED    9274724
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 
            Ther."; date: "1997-05"; volume: "4(5)"; pages: "465-72"
COMMENT     Sequence Label: pBR322-MCK1.35lux
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(292..396)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     464..482
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(520..542)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     642..661
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tctgannnng tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
       61 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
      121 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
      181 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
      241 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
      301 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
      361 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
      421 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
      481 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
      541 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
      601 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
      661 actgagagtg caccatatgg tgaccggatc tcagctgagg tgcaaaaggc tcctgtcata
      721 ttgtgtcctg ctctggtctg ccttccacag cttgggggcc acctagccca cctctcccta
      781 gggatgagag cagccactac gggtctaggc tgcccatgta aggaggcaag gcctggggac
      841 acccgagatg cctggttata attaacccag acatgtggct gccccccccc ccc
//
