LOCUS       sequence-83157-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Baughman JM, Perocchi F, Girgis HS, Plovanich M, Belcher-Timme CA, 
            Sancak Y, Bao XR, Strittmatter L, Goldberger O, Bogorad RL, 
            Koteliansky V, Mootha VK
  TITLE     Integrative genomics identifies MCU as an essential component of the
            mitochondrial calcium uniporter.
  JOURNAL   Nature. 2011 Jun 19;476(7360):341-5. doi: 10.1038/nature10234.
  PUBMED    21685886
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-06-19- 19"; volume: "476"; issue: "7360"; 
            pages: "341-5"
COMMENT     Sequence Label: pDEST40-MCU-V5-HIS
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agctatcaac aagtttgtac
      121 aaaaaagttg gcatggcggc cgccgcaggt agatcgctcc tgctgctcct ctcctctcgg
      181 ggcggcggcg gcgggggcgc cggcggctgc ggggcgctga ctgccggctg cttccctggg
      241 ctgggcgtca gccgccaccg gcagcagcag caccaccgga cggtacacca gaggatcgct
      301 tcctggcaga atttgggagc tgtttattgc agcactgttg tgccctctga tgatgttaca
      361 gtggtttatc aaaatgggtt acctgtgata tctgtgaggc taccatcccg gcgtgaacgc
      421 tgtcagttca cactcaagcc tatctctgac tctgttggtg tatttttacg acaactgcaa
      481 gaagaggatc ggggaattga cagagttgct atctattcac cagatggtgt tcgcgttgct
      541 gcttcaacag gaatagacct cctcctcctt gatgacttta agctggtcat taatgactta
      601 acataccacg tacgaccacc aaaaagagac ctcttaagtc atgaaaatgc agcaacgctg
      661 aatgatgtaa agacattggt ccagcaacta tacaccacac tgtgcattga gcagcaccag
      721 ttaaacaagg aaagggagct tattgaaaga ctagaggatc tcaaagagca gctggctccc
      781 ctggaaaagg tacgaattga gattagcaga aaagctgaga agaggaccac tttggtgcta
      841 tggggtggcc ttgcctacat ggccacacag tttggcattt tggcccggct tacctggtgg
      901 gaatattcct gggacatcat ggagccagta acatacttca tcacttatgg aagtgccatg
      961 gcaatgtatg catattttgt aatgacacgc caggaatatg tttatccaga agccagagac
     1021 agacaatact tactattttt ccataaagga gcc
//
