LOCUS       sequence-83159-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human MCU in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Sancak Y, Markhard AL, Kitami T, Kovacs-Bogdan E, Kamer KJ, Udeshi 
            ND, Carr SA, Chaudhuri D, Clapham DE, Li AA, Calvo SE, Goldberger O,
            Mootha VK
  TITLE     EMRE is an Essential Component of the Mitochondrial Calcium 
            Uniporter Complex.
  JOURNAL   Science. 2013 Nov 14.
  PUBMED    24231807
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Nov 14."
COMMENT     Sequence Label: pLYS5-MCU-Flag
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..42)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgagaattcg gtaccttact tgtcatcgtc atccttgtaa tccatagatc caccagatcc
       61 accagatcca cctctagaat ctttttcacc aatttgtcgg agaggcagat gtacttgtaa
      121 tgggtctctc agtctcttaa ggtccatttc tgcctgagca attgcatcct tgagttgatt
      181 gtatttctct aggtcaaaac gtgacttttt ggctccttta tggaaaaata gtaagtattg
      241 tctgtctctg gcttctggat aaacatattc ctggcgtgtc attacaaaat atgcatacat
      301 tgccatggca cttccataag tgatgaagta tgttactggc tccatgatgt cccaggaata
      361 ttcccaccag gtaagccggg ccaaaatgcc aaactgtgtg gccatgtagg caaggccacc
      421 ccatagcacc aaagtggtcc tcttctcagc ttttctgcta atctcaattc gtaccttttc
      481 caggggagcc agctgctctt tgagatcctc tagtctttca ataagctccc tttccttgtt
      541 taactggtgc tgctcaatgc acagtgtggt gtatagttgc tggaccaatg tctttacatc
      601 attcagcgtt gctgcatttt catgacttaa gaggtctctt tttggtggtc gtacgtggta
      661 tgttaagtca ttaatgacca gcttaaagtc atcaaggagg aggaggtcta ttcctgttga
      721 agcagcaacg cgaacaccat ctggtgaata gatagcaact ctgtcaattc cccgatcctc
      781 ttcttgcagt tgtcgtaaaa atacaccaac agagtcagag ataggcttga gtgtgaactg
      841 acagcgttca cgccgggatg gtagcctcac agatatcaca ggtaacccat tttgataaac
      901 cactgtaaca tcatcagagg gcacaacagt gctgcaataa acagctccca aattctgcca
      961 ggaagcgatc ctctggtgta ccgtccggtg gtgctgct
//
