LOCUS       sequence-8316-ex        4067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Tessier PM, Lindquist S
  TITLE     Prion recognition elements govern nucleation, strain specificity and
            species barriers.
  JOURNAL   Nature. 2007 May 31. 447(7144):556-61.
  PUBMED    17495929
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 May 31. 447(7144):556-61."
COMMENT     Sequence Label: Sup35 CaNM K227C His7
FEATURES             Location/Qualifiers
     source          1..4067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             73..95
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             976..996
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(1068..1086)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1082..1129
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1376..1393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1547..2135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1627..1646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2306..3097,3098..3166))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2929..2948
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3167..3271)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3339..3357
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3395..3417)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3517..3536
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3554..4067)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3749..3770)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3961..3980
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggggatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc
       61 tctagaaata attttgttta actttaagaa ggagatatac atatgtctga ccaacagaat
      121 actcaggacc aattgtctgg tgctatggcc aatgcatctt tgaatggaga tcaatccaag
      181 caacagcaac agcaacaaca acagcaacaa caaaactact acaatccaaa tgccgctcaa
      241 tcttttgttc cacaaggtgg ctaccaacag ttccaacagt tccagcctca acagcagcaa
      301 cagcaatatg gtggatataa tcaatacaac caataccaag gaggttatca acaaaactat
      361 aacaatagag ggggatacca acaaggttat aacaacagag gtggatacca gcaaaactac
      421 aataaccgtg gtggctatca aggatataac caaaaccaac aatatggagg atatcagcaa
      481 tacaattcgc aacctcaaca acaacagcaa caacaatctc aaggtatgtc gttggcagat
      541 ttccaaaaac aaaaaactga acaacaagct tctttgaata aaccagctgt taagaaaacc
      601 cttaaattgg caggatcttc aggtataaaa ttagcaaatg ctacaaagaa agtggatacc
      661 actagcaaac cacaatctaa agaatcatct ccagctccag ctccagctcc agctgctttc
      721 gcttctcaag aagaaaagaa ggaagaaaaa gaagctgctg ctgctgctcc tgaaaccaaa
      781 tgtgaaactt ccgcaccagc agaaaccaaa aaagaagcta ctccaactcc tgctgctaaa
      841 aaggaatcaa ccccaactcc tgctactgct actaagaaag agtcaactcc agtttcaaac
      901 tctgcttctg tggcaactgc agatgctctt gttaaagaac aagaagatga aattgacgaa
      961 gaagttgtca aggatcacca ccatcaccat catcactaag aattcatcga tgatatcaga
     1021 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     1081 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     1141 aactatatcc ggatcggata tcacaggacg ggtgtggtcg ccatgatcgc gtagtcgata
     1201 gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct
     1261 ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagagg ctgcattaat
     1321 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1381 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1441 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1501 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1561 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1621 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1681 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1741 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1801 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1861 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1921 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1981 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2041 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2101 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2161 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2221 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2281 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2341 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2401 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2461 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2521 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2581 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2641 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2701 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2761 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2821 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2881 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2941 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3001 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3061 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3121 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3181 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3241 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     3301 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     3361 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     3421 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     3481 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     3541 gagtgcagta cccaccccgg ttgataatca gaaaagcccc aaaaacagga agattgtata
     3601 agcaaatatt taaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     3661 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3721 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     3781 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     3841 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3901 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3961 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4021 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgt
//
