LOCUS       sequence-8318-ex        3998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Tessier PM, Lindquist S
  TITLE     Prion recognition elements govern nucleation, strain specificity and
            species barriers.
  JOURNAL   Nature. 2007 May 31. 447(7144):556-61.
  PUBMED    17495929
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 May 31. 447(7144):556-61."
COMMENT     Sequence Label: Sup35 Sc/Ca NM Chimera S17R His7 Cys
FEATURES             Location/Qualifiers
     source          1..3998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             73..95
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             907..924
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(999..1017)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1013..1060
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1307..1324)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1478..2066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1558..1577)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2237..3028,3029..3097))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2860..2879
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3098..3202)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3270..3288
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3326..3348)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3448..3467
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3485..3998)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3680..3701)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3892..3911
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggggatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc
       61 tctagaaata attttgttta actttaagaa ggagatatac atatgtcgga ttcaaaccaa
      121 ggcaacaatc agcaaaacta ccagcaatac cgtcagaacg gtaaccaaca acaaggtaac
      181 aacagatacc aaggttatca agcttacaat gctcaagctc aatcttttgt tccacaaggt
      241 ggctaccaac agttccaaca gttccagcct caacagcagc aacagcaata tggtggatat
      301 aatcaataca accaatacca aggaggttat caacaaaact ataacaatag agggggatac
      361 caacaaggtt ataacaacag aggtggatac cagcaaaact acaataaccg tggtggctat
      421 caaggatata accaaaacca acaatatgga ggatatcagc aatacaattc gcaacctcaa
      481 caacaacagc aacaacaatc tcaaggtatg tctttgaacg actttcaaaa gcaacaaaag
      541 caggccgctc ccaaaccaaa gaagactttg aagcttgtct ccagttccgg tatcaagttg
      601 gccaatgcta ccaagaaggt tggcacaaaa cctgccgaat ctgataagaa agaggaagag
      661 aagtctgctg aaaccaaaga accaactaaa gagccaacaa aggtcgaaga accagttaaa
      721 aaggaggaga aaccagtcca gactgaagaa aagacggagg aaaaatcgga acttccaaag
      781 gtagaagacc ttaaaatctc tgaatcaaca cataatacca acaatgccaa tgttaccagt
      841 gctgatgcct tgatcaagga acaggaagaa gaagtggatg acgaagttgt taacgatcat
      901 catgaccatc accatcacca ccattgctaa gaattcatcg atgatatcag atccggctgc
      961 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     1021 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     1081 cggatcggat atcacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca
     1141 agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg
     1201 ggtgcgcata gaaattgcat caacgcatat agcgctagag gctgcattaa tgaatcggcc
     1261 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1321 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1381 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1441 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1501 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1561 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1621 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     1681 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1741 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1801 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1861 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     1921 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1981 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2041 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2101 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2161 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2221 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2281 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2341 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2401 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2461 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2521 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2581 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2641 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2701 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2761 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2821 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     2881 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2941 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3001 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3061 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3121 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3181 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     3241 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     3301 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     3361 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     3421 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcagt
     3481 acccaccccg gttgataatc agaaaagccc caaaaacagg aagattgtat aagcaaatat
     3541 ttaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     3601 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     3661 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     3721 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     3781 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     3841 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     3901 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     3961 cacacccgcc gcgcttaatg cgccgctaca gggcgcgt
//
