LOCUS       sequence-83209-e       15077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length von Willebrand factor precursor ectodomain in 
            mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence 
            and His tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15077)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 15077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: VWF-bio-His
FEATURES             Location/Qualifiers
     source          1..15077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             644..661
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    713..768
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(767..786)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3004..3022)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3090..3194
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3195..3263,3264..4055)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3413..3432)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4226..4814
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4715..4734
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4968..4985
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5102..5123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5138..5143,5144..5161,5162..5168)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5176..5192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5181..5203
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5200..5216
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5200..5216
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5487..5866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5867..6070
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6021..6041
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6067..6091
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     6572..6591
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      6625..6634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8202..8213
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact
       61 caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc
      121 ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac
      181 tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt
      241 ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta
      301 tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg
      361 tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga
      421 ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg
      481 gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc
      541 caaagggttg aattccggca gcctgcacca catcctggac gcccagaaga tgctgtggaa
      601 ccacagggac aggaacctgc ctcctctggc ccctctgggc ccccaccacc atcaccacca
      661 ttgagtctag acacgtgatt aattaaggat cccccgacct cgacctctgg ctaataaagg
      721 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
      781 tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag aggatcgatc
      841 cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg
      901 catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac
      961 acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga
     1021 tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga
     1081 ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg
     1141 gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag
     1201 aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat
     1261 aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta
     1321 tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat
     1381 tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg
     1441 ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca
     1501 gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt
     1561 ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt
     1621 gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg
     1681 gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag
     1741 tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc
     1801 cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag
     1861 tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc
     1921 atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact
     1981 ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat
     2041 aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc
     2101 cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg
     2161 taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg
     2221 ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga
     2281 ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc
     2341 acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat
     2401 atctggatag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     2461 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     2521 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     2581 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag
     2641 attagggtag tatatgctat cctaatttat atctgggtag catatactac ccaaatatct
     2701 ggatagcata tgctatccta atctatatct gggtagcata tgctatccta atctatatct
     2761 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     2821 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     2881 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     2941 gggtagcata tgctatcctc atgataagct gtcaaacatg agaattttct tgaagacgaa
     3001 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     3061 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3121 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3181 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3241 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3301 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3361 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3421 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3481 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3541 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3601 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3661 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3721 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     3781 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3841 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3901 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3961 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4021 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4081 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4141 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4201 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4261 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4321 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     4381 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4441 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4501 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4561 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4621 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4681 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4741 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4801 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4861 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4921 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4981 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     5041 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     5101 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     5161 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     5221 attacgccaa gctctagcta gaggtcgacc aattctcatg tttgacagct tatcatcgca
     5281 gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta
     5341 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
     5401 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     5461 ctcatgtcca atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc
     5521 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     5581 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     5641 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     5701 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
     5761 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
     5821 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     5881 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     5941 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     6001 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     6061 aagcagagct cgtttagtga accgtcagat cctcactctc ttccgcatcg ctgtctgcga
     6121 gggccagctg ttgggctcgc ggttgaggac aaactcttcg cggtctttcc agtactcttg
     6181 gatcggaaac ccgtcggcct ccgaacggta ctccgccacc gagggacctg agcgagtccg
     6241 catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta
     6301 ggctgagcac cgtggcgggc ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc
     6361 tgctgatgat gtaattaaag taggcggtct tgagacggcg gatggtcgag gtgaggtgtg
     6421 gcaggcttga gatccagctg ttggggtgag tactccctct caaaagcggg cattacttct
     6481 gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgatctg
     6541 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
     6601 caggtccaag tttaaactgc ggccgccacc atggtgcctg ctagattcgc tggcgtgctg
     6661 ctggccctgg ccctgatcct gcctggaaca ctgtgtgctg agggcaccag aggcagatcc
     6721 agcaccgcca ggtgcagcct gttcggcagc gacttcgtga acaccttcga cggcagcatg
     6781 tacagcttcg ccggctactg cagctatctg ctggctggag gatgccagaa gcggagcttc
     6841 agcatcatcg gcgacttcca gaacggcaag cgggtgtccc tgagcgtgta cctgggcgag
     6901 ttcttcgaca tccacctgtt cgtgaacggc accgtgaccc agggcgatca gagggtgtcc
     6961 atgccctacg ccagcaaggg cctgtacctg gaaaccgagg ctggctacta caagctgtcc
     7021 ggcgaggcct acggcttcgt ggccagaatc gacggctccg gcaacttcca ggtgctgctg
     7081 tccgaccggt acttcaacaa gacctgtggc ctgtgcggca acttcaacat cttcgccgag
     7141 gacgacttca tgacccagga aggcaccctg accagcgacc cctacgactt cgccaacagc
     7201 tgggccctga gcagcggcga gcagtggtgc gagagagcca gccctcccag cagcagctgc
     7261 aacatcagct ccggcgagat gcagaaaggc ctgtgggagc agtgccagct gctgaagtcc
     7321 acctccgtgt tcgccagatg ccaccccctg gtggaccctg agcccttcgt ggccctgtgc
     7381 gaaaagaccc tgtgcgagtg cgctggcggc ctggaatgtg cctgccctgc cctgctggaa
     7441 tacgcccgga cctgtgccca ggaagggatg gtcctgtacg gctggaccga ccactctgcc
     7501 tgcagccctg tgtgccctgc cggcatggaa taccggcagt gcgtgagccc ctgcgccaga
     7561 acctgtcaga gcctgcacat caacgagatg tgccaggaaa gatgcgtcga cggctgcagc
     7621 tgtcctgagg ggcagctgct ggacgagggc ctgtgtgtgg agagcaccga gtgcccttgc
     7681 gtgcacagcg gcaagagata cccccctggc accagcctga gcagggactg caacacctgt
     7741 atctgccgga acagccagtg gatctgcagc aacgaggaat gcccaggcga gtgtctggtc
     7801 accggccaga gccacttcaa gagcttcgac aacagatact tcaccttcag cggcatctgc
     7861 cagtatctgc tcgcccggga ctgccaggac cacagcttct ccatcgtgat cgagacagtg
     7921 cagtgcgccg acgacagaga tgccgtgtgc accagatccg tgacagtgcg gctgcccggc
     7981 ctgcacaaca gcctggtcaa gctgaagcat ggcgctggcg tggccatgga cggccaggac
     8041 atccagctgc ccctgctgaa gggcgacctg agaatccagc acaccgtgac cgcctccgtg
     8101 agactgagct acggcgagga tctgcagatg gactgggacg gcagaggcag actgctcgtg
     8161 aagctgtccc ccgtgtacgc cggcaagaca tgcggcctgt gtgggaacta caacggcaac
     8221 cagggcgacg actttctgac ccctagcggc ctggccgagc ccagagtgga ggacttcggc
     8281 aacgcctgga agctgcacgg cgattgccag gacctccaga agcagcacag cgacccttgc
     8341 gccctgaacc cccggatgac ccggttcagc gaagaggcct gcgccgtgct gaccagcccc
     8401 accttcgagg cctgtcacag agccgtgagc cccctgccct acctgagaaa ctgcagatac
     8461 gacgtgtgca gctgcagcga cggcagagaa tgtctgtgtg gcgccctggc ttcttatgcc
     8521 gccgcttgtg ccggaagagg cgtgagagtg gcctggcggg agcctggcag atgcgagctg
     8581 aactgcccca agggccaggt gtacctccag tgcggcaccc cctgcaacct gacctgtcgg
     8641 tccctgagct accccgacga agagtgcaac gaggcctgtc tggaaggctg cttctgccct
     8701 cctggactct atatggacga gaggggcgac tgtgtgccta aggcccagtg cccctgttac
     8761 tacgacggcg agatcttcca gcccgaggac atcttcagcg accaccacac catgtgctac
     8821 tgcgaggacg gctttatgca ctgcaccatg agcggcgtgc ctggctctct gctgcccgac
     8881 gctgtgctgt ctagccctct gagccaccgg tccaagcgga gcctgagctg cagacccccc
     8941 atggtcaagc tcgtgtgccc agccgacaat ctgagagccg aggggctgga atgcaccaag
     9001 acctgtcaga actacgacct ggaatgcatg agcatgggct gcgtgagcgg ctgtctgtgc
     9061 cctcctggga tggtccggca cgagaacaga tgcgtggccc tggaacggtg cccatgcttc
     9121 caccagggca aagagtacgc ccctggcgag acagtgaaga tcggctgcaa cacatgcgtg
     9181 tgccgggacc ggaagtggaa ctgcaccgac cacgtgtgcg acgccacctg tagcaccatc
     9241 ggcatggccc actacctgac ctttgacggc ctgaagtacc tgttccccgg cgagtgccag
     9301 tacgtgctgg tgcaggacta ctgcggcagc aaccccggca ccttccggat cctcgtgggc
     9361 aacaagggct gtagccaccc cagcgtgaag tgcaagaaac gcgtgaccat cctggtcgag
     9421 ggcggcgaga tcgagctgtt cgacggcgaa gtgaacgtga agcggcccat gaaggacgag
     9481 acacacttcg aggtggtgga gagcggccgg tacatcatcc tgctgctggg caaggctctg
     9541 agcgtcgtgt gggaccggca cctgagcatc agcgtggtgc tgaagcagac ctaccaggaa
     9601 aaagtctgcg gcctctgcgg caatttcgac ggcatccaga acaacgatct gaccagcagc
     9661 aacctccagg tggaagagga ccccgtggac tttggcaata gctggaaggt gtccagccag
     9721 tgtgccgaca ccagaaaggt gcccctggac agcagccccg ccacctgtca caacaacatc
     9781 atgaagcaga caatggtgga cagctcctgc cggatcctga cctccgacgt gttccaggac
     9841 tgtaacaaac tggtggatcc cgagccttac ctggacgtgt gcatctacga cacctgtagc
     9901 tgcgagagca tcggcgattg cgcctgcttc tgcgacacaa tcgccgccta cgcccatgtg
     9961 tgcgcccagc acggcaaggt ggtcacttgg agaaccgcca ccctgtgtcc ccagagctgc
    10021 gaggaacgga acctgagaga aaacggctac gagtgcgagt ggcggtacaa tagctgcgcc
    10081 cctgcctgcc aggtcacctg tcagcaccct gagcctctgg cctgccccgt gcagtgtgtg
    10141 gagggctgcc acgcccactg tccccctggc aagatcctgg acgagctgct gcagacatgc
    10201 gtggatcccg aggactgtcc cgtgtgcgag gtggccggca gaagattcgc ctccggcaag
    10261 aaagtgaccc tgaaccctag cgaccccgag cactgccaga tctgccactg cgacgtggtc
    10321 aacctgacat gcgaagcctg ccaggaacct ggcggactgg tcgtgcctcc taccgacgcc
    10381 cctgtgtctc ccaccaccct gtacgtggag gacatcagcg agccccccct gcacgacttc
    10441 tactgctccc gcctgctgga cctggtgttc ctgctggacg gcagctctag actgagcgaa
    10501 gccgagttcg aggtgctgaa ggccttcgtg gtggacatga tggaacggct gcggatcagc
    10561 cagaaatggg tccgggtggc cgtggtggag taccacgacg gcagccacgc ctacatcggc
    10621 ctgaaggaca gaaagcggcc cagcgagctg agaagaatcg ccagccaggt caagtacgcc
    10681 ggctcccagg tggccagcac cagcgaagtg ctgaagtaca ccctgttcca gatcttcagc
    10741 aagatcgacc ggcccgaggc ctctagaatc gccctgctgc tgatggccag ccaagaaccc
    10801 cagcggatga gccggaactt cgtgagatac gtgcagggcc tgaagaaaaa gaaagtgatc
    10861 gtgatccccg tgggcatcgg ccctcacgcc aacctgaagc agatccggct gatcgagaag
    10921 caggcccccg agaacaaggc ctttgtgctg tccagcgtgg atgagctgga acagcagcgg
    10981 gacgagatcg tgtcctacct gtgcgatctg gcccctgagg cccctcctcc taccctgcct
    11041 ccccacatgg ctcaggtcac agtgggacct ggactgctgg gcgtgagcac cctgggcccc
    11101 aagcggaaca gcatggtgct ggatgtggcc ttcgtgctcg agggcagcga taagatcggc
    11161 gaggctgact tcaaccggtc caaagaattc atggaagagg tcatccagcg gatggacgtg
    11221 ggccaggaca gcatccacgt gacagtgctc cagtacagct acatggtcac cgtggagtac
    11281 cccttcagcg aggcccagag caagggcgac atcctgcaga gagtgcggga gatcagatac
    11341 cagggcggca accggacaaa tacaggcctg gccctgcgct acctgtccga ccactccttt
    11401 ctggtgtccc agggcgatag agagcaggcc cctaacctgg tgtatatggt cacaggcaac
    11461 cccgccagcg acgagatcaa gagactgccc ggcgacatcc aggtggtgcc tatcggcgtc
    11521 ggccccaacg ccaatgtgca ggaactggaa cggatcggct ggcccaacgc ccccatcctg
    11581 atccaggact tcgagacact gcccagagaa gcccccgacc tggtgctgca gagatgctgt
    11641 agcggcgagg gcctgcagat ccctacactg agccctgccc ccgattgcag ccagcccctg
    11701 gacgtgattc tgctgctgga tgggagcagc agcttccccg ctagctactt cgacgagatg
    11761 aaatctttcg ccaaggcctt tatcagcaag gccaacatcg gccccagact gacccaggtg
    11821 tccgtgctcc agtacggcag catcaccacc atcgacgtgc cttggaacgt ggtgcccgag
    11881 aaggcccatc tgctgtccct ggtggatgtg atgcagagag agggcggacc cagccagatt
    11941 ggcgacgctc tgggcttcgc cgtgagatac ctgacaagcg agatgcacgg agctagacct
    12001 ggagccagca aagccgtggt catcctcgtg accgacgtgt ccgtggatag cgtggatgcc
    12061 gctgccgacg ccgccagatc caacagagtg accgtgttcc ctatcggcat cggcgacaga
    12121 tacgatgccg cccagctgag aattctggcc ggaccagctg gcgacagcaa cgtcgtcaaa
    12181 ctgcagcgga tcgaagatct gcccaccatg gtcaccctgg gcaacagctt tctccacaag
    12241 ctgtgcagcg gcttcgtgcg gatctgcatg gacgaggatg gcaacgagaa gcggccaggc
    12301 gacgtgtgga ccctgcccga ccagtgccac acagtgacct gtcagcccga cggccagacc
    12361 ctgctgaaaa gccacagagt gaactgcgac agaggcctga ggcccagctg tcccaacagc
    12421 cagagccccg tgaaggtgga ggaaacatgc ggctgccgct ggacctgtcc ctgtgtgtgc
    12481 accggaagca gcaccagaca catcgtgaca ttcgatggcc agaacttcaa gctgaccggc
    12541 agctgctcct acgtgctgtt ccagaacaaa gaacaggacc tggaagtgat cctgcacaac
    12601 ggcgcctgtt ctccaggcgc cagacagggc tgcatgaagt ctatcgaagt gaagcacagc
    12661 gccctgtccg tggagctgca cagcgacatg gaagtgaccg tgaatggccg gctggtgtct
    12721 gtgccttacg tgggcggcaa tatggaagtg aatgtgtacg gcgccatcat gcacgaagtg
    12781 cggttcaacc acctgggcca catcttcaca ttcacccccc agaacaatga gttccagctc
    12841 cagctgagtc ccaagacctt cgccagcaag acctacgggc tgtgcgggat ctgtgacgag
    12901 aacggcgcca acgactttat gctgcgggac ggcacagtga ccaccgactg gaaaaccctg
    12961 gtccaggaat ggaccgtcca gaggcctggc cagacatgcc agcccatcct ggaagaacag
    13021 tgcctggtgc ccgacagcag ccactgtcag gtcctgctgc tccccctgtt cgccgagtgt
    13081 cacaaggtgc tggcccctgc caccttctac gccatctgtc agcaggacag ctgccaccag
    13141 gaacaggtct gcgaagtcat tgccagctac gcccacctgt gcagaaccaa cggcgtgtgc
    13201 gtggattggc ggacccccga cttctgcgcc atgagctgcc cccccagcct ggtgtacaac
    13261 cactgcgagc acgggtgccc cagacactgc gacggcaacg tgtccagctg tggcgatcac
    13321 cccagcgagg gctgcttttg ccctcccgac aaagtgatgc tggaaggctc ctgcgtgccc
    13381 gaggaagcct gcacccaatg catcggagag gacggcgtgc agcaccagtt tctggaagcc
    13441 tgggtcccag atcaccagcc ttgtcagatc tgcacctgtc tgagcggccg gaaagtgaac
    13501 tgtaccaccc agccctgtcc taccgccaag gcccccacat gcggactgtg cgaagtggcc
    13561 cggctgagac agaacgccga ccagtgttgc cccgagtacg agtgcgtgtg cgaccccgtg
    13621 tcctgcgatc tgcctcccgt gcctcactgc gagagaggac tgcagcctac cctgaccaat
    13681 cctggcgagt gccggcccaa cttcacctgt gcctgccgga aagaggaatg caagagagtc
    13741 agccccccta gctgccctcc tcaccggctg cccaccctga gaaagaccca gtgctgcgac
    13801 gagtacgaat gcgcctgcaa ctgcgtgaac tctacagtgt cctgccctct gggctacctg
    13861 gcctccaccg ccaccaatga ctgcggatgc accaccacca catgcctgcc cgataaagtg
    13921 tgcgtgcacc ggtccaccat ctaccccgtg ggccagttct gggaagaggg ctgtgacgtg
    13981 tgcacctgta ccgatatgga agatgccgtg atgggcctga gggtggccca gtgcagccag
    14041 aagccctgcg aggactcctg cagatctggc ttcacatacg tgctgcacga gggcgagtgc
    14101 tgcggcagat gcctgcctag cgcctgcgag gtcgtgacag gcagccccag aggcgatagc
    14161 cagtcatctt ggaagtccgt gggcagccag tgggccagcc ctgagaaccc ctgcctgatc
    14221 aacgagtgtg tgcgcgtgaa agaagaggtg ttcatccagc agcggaacgt gtcctgtcct
    14281 cagctcgagg tgcccgtgtg ccctagcggc ttccagctgt catgtaagac cagcgcctgc
    14341 tgccccagct gtagatgcga gcggatggaa gcctgtatgc tgaatggcac agtgatcggc
    14401 ccaggcaaga ccgtgatgat cgatgtgtgc accacctgtc ggtgcatggt ccaggtcggc
    14461 gtgatctccg gcttcaagct ggaatgtaga aagaccacct gtaacccctg ccccctgggg
    14521 tacaaagaag aaaacaacac cggcgagtgt tgtggccggt gtctccccac cgcttgcacc
    14581 attcagctga gaggcggcca gatcatgacc ctgaagcggg acgaaaccct gcaggacggc
    14641 tgcgacaccc acttttgcaa agtgaacgag cggggcgagt acttctggga gaagagagtg
    14701 accggctgtc cccccttcga cgagcacaag tgtctggccg agggcggcaa gattatgaag
    14761 atccccggca catgctgcga cacttgcgag gaacccgagt gcaatgacat caccgccagg
    14821 ctgcagtacg tgaaagtggg cagctgcaag agcgaggtgg aggtggacat ccactactgc
    14881 cagggcaagt gcgcctccaa ggccatgtac agcatcgaca tcaacgacgt gcaggaccag
    14941 tgcagctgtt gcagccccac cagaaccgag cctatgcagg tcgccctgca ctgtaccaac
    15001 ggcagcgtgg tgtaccacga ggtcctgaac gccatggaat gcaagtgcag cccccggaag
    15061 tgcagcaaag gcgcgcc
//
