LOCUS       sequence-83214-e        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chloride-conducting Channelrhodopsin with slow kinetics, 
            neuron-specific promoter, plus red fluorescent protein, excitatory 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Wietek J, Wiegert JS, Adeishvili N, Schneider F, Watanabe H, Tsunoda
            SP, Vogt A, Elstner M, Oertner TG, Hegemann P
  TITLE     Conversion of Channelrhodopsin into a Light-Gated Chloride Channel.
  JOURNAL   Science. 2014 Mar 27.
  PUBMED    24674867
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2014 Mar 27."
COMMENT     Sequence Label: pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             58..984
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIRMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSNIGCIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             994..1047
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1063..1072
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1067..1076
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gggcaggcga gtggccccta gttctggggg cagctcgaat tcaagctgct agccaccatg
       61 gattatggag gcgccctgag tgccgttggg cgcgagctgc tatttgtaac gaacccagta
      121 gtcgtcaatg gctctgtact tgtgcctgag gaccagtgtt actgcgcggg ctggattgag
      181 tcgcgtggca caaacggtgc ccaaacggcg tcgaacgtgc tgcaatggct tgctgctggc
      241 ttctccatcc tactgcttat gttttacgcc taccaaacat ggaagtcaac ctgcggctgg
      301 gaggagatct atgtgtgcgc tatcaggatg gtcaaggtga ttctcgagtt cttcttcgag
      361 tttaagaacc cgtccatgct gtatctagcc acaggccacc gcgtccagtg gttgcgttac
      421 gccgagtggc ttctcacctg cccggtcatt ctcattcacc tgtcaaacct gacgggcttg
      481 tccaacgact acagcaggcg caccatgggt ctgcttgtgt ctaatattgg ctgtattgtg
      541 tggggcgcca cttccgccat ggccaccgga tacgtcaagg tcatcttctt ctgcctgggt
      601 ctgtgttatg gtgctaacac gttctttcac gctgccaagg cctacatcga gggttaccac
      661 accgtgccga agggccggtg tcgccaggtg gtgactggca tggcttggct cttcttcgta
      721 tcatggggta tgttccccat cctgttcatc ctcggccccg agggcttcgg cgtcctgagc
      781 gtgtacggct ccaccgtcgg ccacaccatc attgacctga tgtcgaagaa ctgctggggt
      841 ctgctcggcc actacctgcg cgtgctgatc cacgagcata tcctcatcca cggcgacatt
      901 cgcaagacca ccaaattgaa cattggtggc actgagattg aggtcgagac gctggtggag
      961 gacgaggccg aggctggcgc ggtacccgcg gccgagggca gaggaagtct tctaacatgc
     1021 ggtgacgtgg aggagaatcc cggccctgca ccgggatccg cggccgccat ggtggcctcc
     1081 t
//
