LOCUS       sequence-83215-e       13310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length Coagulation factor V precursor ectodomain in 
            mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence 
            and His tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13310)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 13310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: F5-bio-His
FEATURES             Location/Qualifiers
     source          1..13310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             644..661
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    713..768
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(767..786)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3004..3022)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3090..3194
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3195..3263,3264..4055)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3413..3432)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4226..4814
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4715..4734
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4968..4985
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5102..5123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5138..5143,5144..5161,5162..5168)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5176..5192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5181..5203
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5200..5216
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5200..5216
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5487..5866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5867..6070
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6021..6041
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6067..6091
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     6572..6591
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      6625..6634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact
       61 caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc
      121 ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac
      181 tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt
      241 ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta
      301 tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg
      361 tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga
      421 ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg
      481 gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc
      541 caaagggttg aattccggca gcctgcacca catcctggac gcccagaaga tgctgtggaa
      601 ccacagggac aggaacctgc ctcctctggc ccctctgggc ccccaccacc atcaccacca
      661 ttgagtctag acacgtgatt aattaaggat cccccgacct cgacctctgg ctaataaagg
      721 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
      781 tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag aggatcgatc
      841 cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg
      901 catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac
      961 acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga
     1021 tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga
     1081 ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg
     1141 gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag
     1201 aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat
     1261 aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta
     1321 tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat
     1381 tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg
     1441 ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca
     1501 gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt
     1561 ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt
     1621 gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg
     1681 gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag
     1741 tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc
     1801 cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag
     1861 tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc
     1921 atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact
     1981 ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat
     2041 aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc
     2101 cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg
     2161 taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg
     2221 ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga
     2281 ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc
     2341 acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat
     2401 atctggatag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     2461 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     2521 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     2581 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag
     2641 attagggtag tatatgctat cctaatttat atctgggtag catatactac ccaaatatct
     2701 ggatagcata tgctatccta atctatatct gggtagcata tgctatccta atctatatct
     2761 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     2821 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     2881 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     2941 gggtagcata tgctatcctc atgataagct gtcaaacatg agaattttct tgaagacgaa
     3001 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     3061 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3121 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3181 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3241 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3301 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3361 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3421 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3481 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3541 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3601 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3661 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3721 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     3781 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3841 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3901 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3961 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4021 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4081 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4141 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4201 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4261 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4321 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     4381 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4441 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4501 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4561 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4621 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4681 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4741 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4801 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4861 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4921 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4981 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     5041 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     5101 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     5161 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     5221 attacgccaa gctctagcta gaggtcgacc aattctcatg tttgacagct tatcatcgca
     5281 gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta
     5341 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
     5401 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     5461 ctcatgtcca atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc
     5521 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     5581 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     5641 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     5701 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
     5761 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
     5821 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     5881 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     5941 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     6001 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     6061 aagcagagct cgtttagtga accgtcagat cctcactctc ttccgcatcg ctgtctgcga
     6121 gggccagctg ttgggctcgc ggttgaggac aaactcttcg cggtctttcc agtactcttg
     6181 gatcggaaac ccgtcggcct ccgaacggta ctccgccacc gagggacctg agcgagtccg
     6241 catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta
     6301 ggctgagcac cgtggcgggc ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc
     6361 tgctgatgat gtaattaaag taggcggtct tgagacggcg gatggtcgag gtgaggtgtg
     6421 gcaggcttga gatccagctg ttggggtgag tactccctct caaaagcggg cattacttct
     6481 gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgatctg
     6541 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
     6601 caggtccaag tttaaactgc ggccgccacc atggcccctg gctgccctag actgtgggtg
     6661 ctggtcgtgc tgggcacatc ttgggtcggc tggggctctc agggaacaga ggccgcccag
     6721 ctgagacagt tctacgtggc cgcccagggc atcagctggt cctaccggcc cgagcccacc
     6781 aacagcagcc tgaacctgag cgtgaccagc ttcaagaaaa tcgtgtaccg ggagtacgag
     6841 ccctacttca agaaagagaa gccccagagc accatctctg gcctgctggg ccctacactg
     6901 tacgccgaag tgggcgacat catcaaggtg cacttcaaga acaaggccga caagcccctg
     6961 agcatccacc ctcagggcat ccggtacagc aagctgtccg agggcgccag ctacctggat
     7021 cacaccttcc ccgccgagaa gatggatgat gccgtggccc caggcagaga atacacctac
     7081 gagtggagca tcagcgagga cagcggcccc acccacgacg atcccccctg cctgacccac
     7141 atctactaca gccacgagaa cctgatcgag gacttcaaca gcggcctgat cggccccctg
     7201 ctgatctgca agaagggcac cctgacagag ggcggcacac agaaaacctt cgacaagcag
     7261 atcgtgctgc tgttcgccgt gttcgacgag agcaagagct ggtcccagag cagctccctg
     7321 atgtacaccg tgaacggcta cgtgaacggc accatgcccg acatcaccgt gtgcgcccac
     7381 gaccacatca gctggcatct gctgggcatg agcagcggcc ctgagctgtt cagcatccac
     7441 ttcaacggcc aggtgctgga acagaaccac cacaaggtgt ccgccatcac cctggtgtct
     7501 gccacctcca ccaccgccaa catgacagtg ggccccgagg gcaagtggat catctccagc
     7561 ctgaccccca agcatctgca ggccggaatg caggcctaca tcgacatcaa gaactgcccc
     7621 aagaaaaccc ggaacctgaa gaagatcacc cgggagcagc ggcggcacat gaagagatgg
     7681 gagtacttta tcgccgccga ggaagtgatc tgggactacg cccccgtgat ccccgccaat
     7741 atggacaaga agtaccggtc ccagcacctg gacaacttca gcaaccagat cggcaagcac
     7801 tacaagaaag tgatgtacac acagtacgag gacgagagct tcaccaagca caccgtgaat
     7861 cccaacatga aagaggacgg catcctgggc cccatcatca gagcccaggt ccgggacacc
     7921 ctgaagatcg tgttcaagaa tatggccagc cggccctaca gcatctaccc ccacggcgtg
     7981 accttcagcc cctacgagga tgaagtgaac agcagcttca ccagcggccg gaacaacacc
     8041 atgatccggg ccgtgcagcc tggcgaaacc tacacataca agtggaacat cctggaattc
     8101 gacgagccca ccgagaacga cgcccagtgc ctgaccagac cctactacag cgacgtggac
     8161 atcatgcggg atatcgcctc cggcctgatt gggctgctgc tgatttgcaa aagccggtcc
     8221 ctggacagaa gaggcatcca gagagccgcc gacatcgagc agcaggccgt gttcgctgtg
     8281 tttgatgaga acaagtcctg gtatctggaa gataacatca acaagttctg cgagaacccc
     8341 gacgaagtga agcgcgacga ccccaagttc tacgagtcca acatcatgtc caccatcaat
     8401 ggctacgtgc ccgagagcat caccaccctg ggcttttgct tcgacgacac cgtgcagtgg
     8461 cacttttgca gcgtcggcac ccagaacgag atcctgacca tccactttac cggccacagc
     8521 ttcatctacg gcaagcggca cgaggatacc ctgaccctgt tccccatgag aggcgagagc
     8581 gtgaccgtga ccatggacaa cgtgggcacc tggatgctga ccagcatgaa cagctccccc
     8641 cggtccaaga agctgcggct gaagttccgg gacgtgaagt gcatccccga cgacgacgag
     8701 gacagctacg agatcttcga gccccccgag agcaccgtga tggccacccg gaagatgcac
     8761 gaccggctgg aacccgagga tgaggaaagc gacgccgact acgactacca gaaccggctg
     8821 gccgctgccc tgggcatcag aagcttccgg aactcctccc tgaaccagga agaggaagag
     8881 ttcaacctga ccgccctggc cctggaaaac ggcaccgagt tcgtgagcag caacaccgac
     8941 atcatcgtgg gcagcaacta cagcagcccc agcaacatct ccaagttcac cgtgaacaac
     9001 ctggccgagc ctcagaaagc ccctagccat cagcaggcca ccacagccgg aagccccctg
     9061 agacacctga tcggcaagaa cagcgtgctg aactccagca ccgccgagca cagcagccct
     9121 tacagcgaag atcccatcga agatcccctg cagcctgacg tgaccggcat cagactgctg
     9181 tctctgggag ccggcgagtt caagagccag gaacacgcca agcacaaggg ccccaaggtg
     9241 gagagagatc aggccgccaa gcaccggttc agctggatga agctgctggc ccacaaagtg
     9301 ggcagacacc tgagccagga cacaggcagc cctagcggca tgaggccctg ggaggatctg
     9361 cctagccagg ataccggctc ccccagcaga atgcggccct ggaaggaccc tcccagcgac
     9421 ctcctgctgc tgaagcagag caacagcagc aagatcctcg tgggcagatg gcacctggcc
     9481 agcgagaagg gctcctacga gatcatccag gacaccgatg aggacaccgc cgtgaacaat
     9541 tggctgatta gcccccagaa cgcctctaga gcctggggcg agagcacccc cctggccaac
     9601 aagcctggca agcagagcgg ccaccccaag tttcccagag tgcggcacaa gagcctccag
     9661 gtccgccagg atggaggaaa gagccggctg aagaagtccc agttcctgat caagacccgg
     9721 aagaagaaga aagaaaagca cacccaccac gcccctctgt cccccagaac cttccacccc
     9781 ctgcggagcg aggcctacaa caccttcagc gagcggcggc tgaagcacag cctggtgctg
     9841 cacaagtcca acgagacaag cctgcccact gacctgaatc agaccctccc cagcatggac
     9901 ttcggctgga tcgccagcct gcccgaccac aaccagaaca gctccaacga caccggccag
     9961 gcctcttgtc ctcccggcct gtaccagacc gtgccccccg aggaacacta ccagacattc
    10021 ccaatccaag accccgacca gatgcatagc accagcgacc ccagccacag aagcagcagc
    10081 cccgaactga gcgagatgct ggaatacgac cggtcccaca agagcttccc caccgacatc
    10141 tcccagatga gccccagcag cgagcacgaa gtgtggcaga cagtgatctc acccgacctc
    10201 tctcaggtca ccctgagccc cgagctgtct cagacaaacc tgagccctga cctgtcccac
    10261 accaccctct ccccagagct gatccagaga aacctctctc ccgccctggg ccagatgccc
    10321 atcagccctg atctgtccca tacaacactg tctcccgatc tgagccatac cacactcagc
    10381 ctggacctgt cacagaccaa tctgtcaccc gaactgagtc agacaaatct cagccccgct
    10441 ctcggacaga tgcctctgtc acctgatctc tctcacacca ctctgagtct ggacttcagc
    10501 cagacaaacc tcagtccaga actctcccac atgaccctgt caccagaact gtcccagact
    10561 aatctgagtc ctgcactcgg gcagatgcct atctccccag atctcagtca tacaaccctg
    10621 tccctggatt tctcacagac taacctctca cctgaactga gccagactaa cctgtctcca
    10681 gctctcgggc agatgcctct gagccccgat cccagccaca ctactctgtc tctggacctc
    10741 tctcagacaa atctgtcccc agaactgtca cagacaaacc tcagtcccga tctgagcgaa
    10801 atgcccctgt tcgccgacct cagccagatc cccctgaccc ccgatctgga tcagatgacc
    10861 ctgagtccag acctgggcga gacagacctg agccccaact tcggccagat gagtctgtct
    10921 ccagacctga gtcaggtcac actgtcacct gacatcagcg ataccaccct gctgccagat
    10981 ctgtctcaga tttctcctcc acctgatctc gatcagatct tctaccccag cgagagcagc
    11041 cagagcctgc tgctgcagga attcaacgag tccttcccct accctgacct gggacagatg
    11101 ccaagcccta gcagccccac cctgaacgac acctttctga gcaaagaatt caaccccctc
    11161 gtgatcgtgg gcctgagcaa ggacggcacc gactacatcg agatcattcc caaagaagag
    11221 gtgcagagca gcgaggacga ctacgccgag atcgactacg tgccctacga cgacccctac
    11281 aagaccgacg tgcggaccaa catcaactcc agccgggacc ccgataatat cgccgcttgg
    11341 tatctgcgga gcaacaacgg caaccggcgg aactactaca ttgccgccga agagatcagc
    11401 tgggactaca gcgagttcgt gcagcgggag acagacatcg aggactccga cgacatcccc
    11461 gaggacacca cctacaaaaa ggtggtgttc cggaagtacc tggacagcac ctttaccaag
    11521 cgggacccca gaggcgagta cgaggaacac ctgggcattc tgggccctat tatccgggcc
    11581 gaggtggacg atgtgatcca ggtccgcttc aagaacctgg cctccaggcc ttactctctg
    11641 cacgcccacg gcctgagcta cgagaagtcc agcgagggca agacctatga ggacgactcc
    11701 cccgagtggt tcaaagagga caacgccgtc cagcccaaca gctcctacac ctatgtgtgg
    11761 cacgccaccg agagaagcgg acctgagagc cctggctctg cctgcagagc ctgggcctac
    11821 tactctgccg tgaaccccga gaaggacatc cactccgggc tgatcggacc tctgctcatt
    11881 tgtcagaagg gcatcctgca caaggacagc aacatgcccg tggacatgcg ggagttcgtc
    11941 ctgctgttca tgaccttcga cgagaagaag tcttggtact acgagaagaa aagcagaagc
    12001 agctggcggc tgaccagctc cgagatgaag aagtctcacg agttccacgc catcaacggc
    12061 atgatctact ccctgcccgg cctgaagatg tacgagcagg aatgggtccg gctgcatctg
    12121 ctgaacatcg gcggcagcca ggatatccac gtggtgcact ttcacggcca gaccctgctg
    12181 gaaaatggca acaagcagca ccagctgggc gtgtggcctc tgctgcccgg ctccttcaag
    12241 accctggaaa tgaaggccag caagcccggg tggtggctgc tgaacaccga agtgggggag
    12301 aaccagaggg ccgggatgca gacccccttc ctgatcatgg accgggactg cagaatgcct
    12361 atgggcctgt ccaccggcat catcagcgac agccagatca aggccagcga gttcctgggc
    12421 tactgggagc ccagactggc cagactgaat aacggcggca gctacaacgc ttggagcgtg
    12481 gagaagctgg ccgccgagtt cgcctccaag ccctggattc aggtggacat gcagaaagaa
    12541 gtgatcatca ccggcatcca gacccagggc gccaagcatt acctgaagtc ctgctacacc
    12601 accgagtttt acgtggccta cagcagcaat cagatcaact ggcagatctt caagggcaac
    12661 agcacccgga acgtgatgta ctttaacggc aacagcgacg ccagcaccat caaagagaac
    12721 cagttcgacc cccccatcgt ggcccggtac atccggatca gccccaccag agcctacaac
    12781 cggcccaccc tgagactgga actgcagggc tgcgaagtga acggctgcag cacccctctg
    12841 ggcatggaaa acgggaagat cgagaataag cagatcaccg ccagcagctt taagaaatct
    12901 tggtggggcg actactggga acccttccgg gccaggctga atgcccaggg cagagtgaac
    12961 gcctggcagg ccaaggccaa caacaacaag cagtggctgg aaatcgatct gctgaagatc
    13021 aagaagatta ccgccatcat cacccagggc tgcaagagcc tgagcagcga gatgtacgtg
    13081 aagtcctaca ccatccacta ctccgagcag ggcgtggagt ggaagcccta ccggctgaag
    13141 tccagcatgg tggacaagat ctttgagggc aacaccaaca ccaagggcca cgtgaagaac
    13201 tttttcaacc cccctatcat cagccggttc atcagagtga tccccaagac ctggaaccag
    13261 agcattgccc tgcggctgga actgttcggc tgcgacatct atggcgcgcc
//
