LOCUS       sequence-83222-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  TITLE     TLR Signaling Plasmids
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFlag-CMV1-hMD2
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..72
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             73..96
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(761..780)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 ttagtgaacc gtcagaatta attcaccatg tctgcacttc tgatcctagc tcttgttgga
       61 gctgcagttg ctgactacaa agacgatgac gacaagcttg cggccgcgga agctcagaag
      121 cagtattggg tctgcaactc atccgatgca agtatttcat acacctactg tgataaaatg
      181 caatacccaa tttcaattaa tgttaacccc tgtatagaat tgaaaggatc caaaggatta
      241 ttgcacattt tctacattcc aaggagagat ttaaagcaat tatatttcaa tctctatata
      301 actgtcaaca ccatgaatct tccaaagcgc aaagaagtta tttgccgagg atctgatgac
      361 gattactctt tttgcagagc tctgaaggga gagactgtga atacaacaat atcattctcc
      421 ttcaagggaa taaaattttc taagggaaaa tacaaatgtg ttgttgaagc tatttctggg
      481 agcccagaag aaatgctctt ttgcttggag tttgtcatcc tacaccaacc taattcaaat
      541 tagtcgactc tagaggatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
      601 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
      661 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
      721 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
      781 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
      841 ctgcctcagc ctcccgagtt gttgggattc c
//
