LOCUS       sequence-83223-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Jinek M, East A, Cheng A, Lin S, Ma E, Doudna J
  TITLE     RNA-programmed genome editing in human cells.
  JOURNAL   elife. 2013;2:e00471. doi: 10.7554/eLife.00471. Epub 2013 Jan 29.
  PUBMED    23386978
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "elife."; 
            date: "2013"; volume: "2:e00471. doi"; pages: " 10.7554/eLife.00471.
            Epub 2013 Jan 29"
COMMENT     Sequence Label: pMJ920
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      120..129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccctacttc caatccaatg
      121 ccaccatgga caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg
      181 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
      241 accggcacag catcaagaag aacctgatcg gcgccctgct gttcgacagc ggagaaacag
      301 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
      361 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
      421 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
      481 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
      541 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
      601 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca
      661 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa
      721 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga
      781 gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg
      841 gcaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg
      901 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc
      961 tggcccaga
//
