LOCUS       sequence-83225-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Jinek M, East A, Cheng A, Lin S, Ma E, Doudna J
  TITLE     RNA-programmed genome editing in human cells.
  JOURNAL   elife. 2013;2:e00471. doi: 10.7554/eLife.00471. Epub 2013 Jan 29.
  PUBMED    23386978
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "elife."; 
            date: "2013"; volume: "2:e00471. doi"; pages: " 10.7554/eLife.00471.
            Epub 2013 Jan 29"
COMMENT     Sequence Label: pMJ920
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(26..739,740..742))
                     /codon_start=1
                     /product="enhanced GFP (EGFP) derivative that exhibits 
                     robust folding and enhanced photostability (Zhong et al., 
                     2019)"
                     /label=SiriusGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(71..92)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     679..700
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(782..802)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(809..829)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(842..868)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(848..874)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctatagaata gggccctcta gattactact tgtacagctc gtccatgccg agagtgatcc
       61 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
      121 tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
      181 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg acgttgtggc
      241 ggatcttgaa gttcgccttg atgccgttct tctgcttgtc ggccgtgata tagacgttgt
      301 ggctgttgaa gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      361 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      421 tcttgtaggt gccgtcgtcc ttgaaggaga tggtgcgctc ctggacgtag ccttcgggca
      481 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
      541 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
      601 agatgaactt cagggtcagc ttgccgttgg tggcatcgcc ctcgccctcg ccgcgcacgc
      661 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
      721 acagctcctc gcccttgctc acggatccgc tgctgccgtt gctcccacta ccaatgccgt
      781 ccactttccg ctttttctta ggatcttcca ccttgcgttt tttcttgggg gagccgctgc
      841 ccaggctggc ataatcgggc acgtcatagg gataggcgtc gcctcccagc tgagacaggt
      901 cgatccgtgt ctcgtacagg ccggtgatgc tctggtggat cagggtggcg tccagcacct
      961 ctttggtgct ggtgtacctc ttccggtc
//
