LOCUS       sequence-83228-e        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Jinek M, East A, Cheng A, Lin S, Ma E, Doudna J
  TITLE     RNA-programmed genome editing in human cells.
  JOURNAL   elife. 2013;2:e00471. doi: 10.7554/eLife.00471. Epub 2013 Jan 29.
  PUBMED    23386978
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "elife."; 
            date: "2013"; volume: "2:e00471. doi"; pages: " 10.7554/eLife.00471.
            Epub 2013 Jan 29"
COMMENT     Sequence Label: pMJ920
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      298..307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(354..373)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(355..373)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(418..621)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(447..467)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        622..1001
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtctgtggaa gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat
       61 agcagatccg gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg
      121 cctcggctgt ttctccgctg tcgaacagca gggcgccgat caggttcttc ttgatgctgt
      181 gccggtcggt gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga
      241 tcacggccca gcccacagag ttggtgccga tgtccaggcc gatgctgtac ttcttgtcca
      301 tggtggcatt ggattggaag tagggatccg agctcggtac caagcttggg tctccctata
      361 gtgagtcgta ttaatttcga taagccagta agcagtgggt tctctagtta gccagagagc
      421 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
      481 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
      541 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
      601 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
      661 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
      721 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
      781 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
      841 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
      901 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
      961 cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgtat atctggc
//
