LOCUS       sequence-83232-e        8209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  xylose-dependent expression of the type-IV agrC and type-I agrA 
            genes of S. aureus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Marchand N, Collins CH
  TITLE     Peptide-based communication system enables Escherichia coli to 
            Bacillus megaterium interspecies signaling.
  JOURNAL   Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975.
            Epub 2013 Jul 5.
  PUBMED    23775238
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24975";
            journalName: "Biotechnol Bioeng"; date: "2013-11"; volume: "110"; 
            issue: "11"; pages: "3003-12"
COMMENT     Sequence Label: pXylA-agrCA-IV
FEATURES             Location/Qualifiers
     source          1..8209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5053..5069)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5053..5069)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5066..5088)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5077..5093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5101..5107,5108..5125,5126..5131))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5146..5167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5284..5301)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5455..6043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5535..5554)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6214..7005,7006..7074))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6837..6856
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7075..7179)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7247..7265
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7303..7325)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7425..7444
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttgatatgcc tcctaaattt ttatctaaag tgaatttagg aggcttactt gtctgctttc
       61 ttcattagaa tcaatccttt tttaaaagtc aatattactg taacataaat atatatttta
      121 aaaatatccc actttatcca attttcgttt gttgaactaa tgggtgcttt agttgaagaa
      181 taaaagacca cattaaaaaa tgtggtcttt tgtgtttttt taaaggattt gagcgtagcg
      241 aaaaatcctt ttctttctta tcttgataat aagggtaact attgccggat cgtcctcagg
      301 agtaggcgac atcgctaaat aatgatctat gcggtgtgaa ataccgcaca gatgcgtaag
      361 gagaaaatac cgcatcaggc gccctttgcg ttcacttaac taacttatag gggtaacact
      421 taaaaaagaa tcaataacga tagaaaccgc tcctaaagca ggtgcatttt ttcctaacga
      481 agaaggcaat agttcacatt tattgtctaa atgagaatgg actctagaag aaacttcgtt
      541 tttaatcgta tttaaaacaa tgggatgaga ttcaattata tgatttctca agataacagc
      601 ttctatatca aatgtattaa ggatattggt taatccaatt ccgatataaa agccaaagtt
      661 ttgaagtgca tttaacattt ctacatcatt tttatttgcg cgttccacaa tctcttttcg
      721 agaaatattc ttttcttctt tagagagcga agccagtaac gctttttcag aagcatataa
      781 ttcccaacag cctcgatttc cacagctgca tttgggtcca ttaaaatcta tcgtcatatg
      841 acccatttcc ccagaaaaac cctgaacacc tttatacaat tcgttgttaa taacaagtcc
      901 agttccaatt ccgatattaa tactgatgta aacgatgttt tcatagtttt ttgtcatacc
      961 aaatactttt tcaccgtatg ctcctgcatt agcttcattt tcaacaaaaa ccggaacatt
     1021 aaactcactc tcaattaaaa actgcaaatc tttgatattc caatttaagt taggcatgaa
     1081 aataatttgc tgatgacgat ctacaaggcc tggaacacaa attcctattc cgactagacc
     1141 ataaggggac tcaggcatat gggttacaaa accatgaata agtgcaaata aaatctcttt
     1201 tacttcacta gcggaagaac tagacaagtc agaagtcttc tcgagaataa tatttccttc
     1261 taagtcggtt agaattccgt taagatagtc gactcctata tcaataccaa tcgagtagcc
     1321 tgcattctta ttaaaaacaa gcattacagg tcttctgccg cctctagatt gccctgcccc
     1381 aatttcaaaa ataaaatctt tttcaagcag tgtatttact tgagaggaga cagtagactt
     1441 gtttaatcct gtaatctcag agagagttgc cctggagaca ggggagttct tcaaaatttc
     1501 atctaatatt aatttttgat tcattttttt tactaaagct tgatctgcaa tttgaataat
     1561 aaccactcct ttgtttatcc accgaactaa gttggtgttt tttgaagctt gaattagata
     1621 tttaaaagta tcatatctaa tattataact aaattttcta aaaaaaacat tgaaataaac
     1681 atttattttg tatatgacga gataaagtta gtttattgga taaacaaact aactcaatta
     1741 agatagttga tggataaact tgttcactta aatcaaaggg ggaaatgaca aatggtccaa
     1801 actagtatgg aattgttaaa cagttacaac tttgttttgt tcgtattaac tcaaataata
     1861 ctaatgttta cagtaccatc aattattagt ggtatcaaat acagtaaatc tgactatttg
     1921 tatatcacgg gtattacggc attatcgtta attctattta attttatcga tagcgtaacc
     1981 ttaataatac taactatatt cataattata ttatatctca gcaaaatcaa atggtattct
     2041 attttgctga taatgacctc gcagattatt ttatactgtg caaattacat gtatatagtt
     2101 atattcacat atattgtcaa aatcgttgat agtatatttg taatatttcc catcttcttt
     2161 gtagtttatg tgacaatcag tatactattt tcttatataa taaatagagt tctcaaaaag
     2221 attagctcaa catatctaat actaaacaaa ggttttttaa tagttatttc aaccattcta
     2281 ctgcttactt tttcattgtt tttcttttat tctcaaataa actcggatga agctaaagta
     2341 ataaggcagt attcttttat ttttattgga atcactatat ttttaagtat attaacattt
     2401 gttatttcac aatttctctt taaagagatg aaatacaaaa gaaatcaaga agaaattgag
     2461 acctattacg aatacacatt aaagattgaa gcaattaata atgaaatgcg taagttccga
     2521 catgattatg tcaatatctt aactacactt tcagaataca ttcgagaaga tgacatgcct
     2581 ggtctacgtg actatttcaa taaaaatata gtgccaatga aagataactt acaaatgaac
     2641 gctattaagt taaatggtat tgagaatctt aaagtacgtg aaattaaagg cttaatcact
     2701 gctaaaattt tacgtgcaca agaaatgagt ataccgatta gtattgaaat accagatgaa
     2761 gtaactcaca ttaatttgaa tatgattgat ttaagtcgca gtattggtat tattcttgat
     2821 aacgcaatag aggcatcaac tgaaattgat gatcctatca ttcgagttgc atttattgaa
     2881 agcgaaaatt cagtaacgtt tattgttatg aataaatgtg cagatgatat accacgtatt
     2941 catgaattgt tccaagaaag tttttctact aaaggtgaag gtcgtggttt aggtctatca
     3001 actttaaaag aaattgctga taatgcagac aatgtcttat tagatacaat tatcgagaat
     3061 ggtttcttta ttcaaaaggt tgaaattatt aacaactaac cataaggatg tgaatgtatg
     3121 aaaattttca tttgcgaaga cgatccaaaa caaagagaaa acatggttac cattattaaa
     3181 aattatataa tgatagaaga aaagcctatg gaaattgccc tcgcaactga taatccttat
     3241 gaggtgcttg agcaagctaa aaatacgaat gacataggct gttacttttt agatattcaa
     3301 ctttcaactg atattaatgg tatcaaatta ggcagtgaaa ttcgtaagca tgatccagtt
     3361 ggtaacatta tatttgttac gagtcacagt gaacttacct atttaacttt tgtctacaaa
     3421 gttgcagcga tggattttat ttttaaagat gatccagctg aattaagaac tcgaattata
     3481 gactgtttag aaactgcaca tacacgctta caattgttgt ctaaagataa tagcgttgaa
     3541 acgattgaat taaaacgtgg cagtaattca gtgtatgttc aatatgatga tattatgttt
     3601 tttgaatcat caacaaaatc tcacagactc attgcccatt tagataaccg ccaaattgaa
     3661 ttttatggta atttaaaaga actgagccaa ttagatgatc gtttctttag atgtcataat
     3721 agctttgtcg tcaatcgcca taatattgaa tctatagatt caaaagagcg aattgtctat
     3781 tttaaaaata aagaacactg ctatgcatca gtgagaaacg ttaaaaaaat ataataagat
     3841 aataaagtca gttaacggcg tattcaattg tggatcctct agagtcattt atattcaact
     3901 gttaactaaa actgtctgca aattaatcaa tgtctctagt aaaattccga ttttataccc
     3961 actcctattt tcatcaatag gacatgatat aataaaaaag aacggagatt gaggcctccg
     4021 ttctcttggt tgtacttgtt gcaatagtac gtttatttgg ttacgtatat tatagcaagt
     4081 actccaacaa aaaatcaatc gttggaggaa tttagaaatg aatggaatta tcaaaacaat
     4141 aaaaacaacg caatatgctc aaatacataa cgcgccatta caaacggacc tggaggactt
     4201 gcggtctatc ggtctattaa gtcatattat gagcttaggt gaaggttgga cgattaggaa
     4261 gacccagtta caaaacaaat tttctcgtag aaatgtagac gctgcttgga aggaattagc
     4321 tattaaacaa tacgctgtcg gttttagcgc atatgttgac ggaaaaaaag attattttta
     4381 cgccgtatca gatattccga tttcgcaagc taatttcgaa ttgcttgtca ttgaacaaat
     4441 aaatctctta caatcgcaag gtaaaaacgt catgacgtta agcgtgatac aacattcgcc
     4501 acttgaaatc actgaaaaaa tatctgatgt acaaaatgta catcagatac aaaataagca
     4561 aatttcttct gatgtacgtt ctgtacaaca cagtgtgtac aacacagaac gtacacctat
     4621 aaataaaaaa gaaacaaatg aaaaattaac aaatgaaaaa attagtagta gtccacgtga
     4681 ttttattgat gataaattac gagaaaaata taataatgta cctttcgacg aagtgaaaag
     4741 tgaaatgctt aacgatactg taatcgttga tacgaataag caatataaat cactattaga
     4801 ataccgtctt aaacattgga aaccgaagca aacaaaaagg aaacgtgtag tacgtaatgc
     4861 acgtaaagaa atggttccaa agtggctaca aacaaatgat gaaaatgcag tagcagaaga
     4921 tcttccagtg aataatgatt ttgaagctga aaaggaaaaa atgaaggcgg agcttatgca
     4981 attagatgca gaattaaaag caggtaatcg ttaataaatg acctgcaggc atgcaagctt
     5041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5101 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5161 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5221 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5281 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5341 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5401 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5461 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5521 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5581 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5641 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5701 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5761 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5821 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5881 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5941 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6001 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6061 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6121 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6181 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6241 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6301 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6361 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6421 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6481 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6541 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6601 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6661 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6721 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6781 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6841 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6901 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6961 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7021 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7081 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7141 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7201 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     7261 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     7321 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     7381 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     7441 tgtactgaga gtgcaccata gatctggagc tgtaatataa aaaccttctt caactaacgg
     7501 ggcaggttag tgacattaga aaaccgactg taaaaagtac agtcggcatt atctcatatt
     7561 ataaaagcca gtcattaggc ctatctgaca attcctgaat agagttcata aacaatcctg
     7621 catgataacc atcacaaaca gaatgatgta cctgtaaaga tagcggtaaa tatattgaat
     7681 tacctttatt aatgaatttt cctgctgtaa taatgggtag aaggtaatta ctattattat
     7741 tgatatttaa gttaaaccca gtaaatgaag tccatggaat aatagaaaga gaaaaagcat
     7801 tttcaggtat aggtgttttg ggaaacaatt tccccgaacc attatatttc tctacatcag
     7861 aaaggtataa atcataaaac tctttgaagt cattctttac aggagtccaa ataccagaga
     7921 atgttttaga tacaccatca aaaattgtat aaagtggctc taacttatcc caataaccta
     7981 actctccgtc gctattgtaa ccagttctaa aagctgtatt tgagtttatc acccttgtca
     8041 ctaagaaaat aaatgcaggg taaaatttat atccttcttg ttttatgttt cggtataaaa
     8101 cactaatatc aatttctgtg gttatactaa aagtcgtttg ttggttcaaa taatgattaa
     8161 atatctcttt tctcttccaa ttgtctaaat caattttatt aaagttcat
//
