LOCUS       sequence-83250-e        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Drosophila Sgf11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Mohan RD, Dialynas G, Weake VM, Liu J, Martin-Brown S, Florens L, 
            Washburn MP, Workman JL, Abmayr SM
  TITLE     Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B 
            ubiquitination and leads to neural and retinal degeneration.
  JOURNAL   Genes Dev. 2014 Feb 1;28(3):259-72. doi: 10.1101/gad.225151.113.
  PUBMED    24493646
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.225151"; journalName: "Genes Dev"; date: "2014-02-1- 
            1"; volume: "28"; issue: "3"; pages: "259-72"
COMMENT     Sequence Label: pBacPak-FLAG-HIS-Sgf11
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..105
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    622..633
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 ctgttttcgt aacagttttg taataaaaaa acctataaat acggatccat gggtcatcat
       61 catcaccatc acgattacgg agactacaag gacgatgatg acaaggagaa cccctacttc
      121 cagggcctcg agatgtctgc agccaacatg ccgacgacaa caggagctca gggatcggga
      181 aaccaagttc ccacaactag cactacgatt gtaaaccact tccgggagtt gatcaaggaa
      241 ccgaagaacc tcgacgaggc ggccaactat ctgtaccagt cgctgctcga cgacgccgta
      301 gtcggaatct ttaacgagac acatcatctg cgaaagtcag ggaatctggc cgccctggac
      361 ggagtgccgg aggactcaac ctatcgaatg tgcgagatgc ccaacctgga catattcggc
      421 atctcaacgg ccaaaaagcc aatggactgc acctgcccca actgtgatcg cctggtggcc
      481 gccgcccgct tcgccccgca cctggaaaag tgcatgggca tgggtcgtat ctcgtcgcga
      541 attgcctctc gtcgcttggc caccaaggag ggcgccacat ccgcccacct gcactcttcg
      601 ggaaataccg gaggcactga tgatgaggat gacgtggact ggtcgtccga caagcgtcga
      661 aagaaatcaa accagaactc aaggaacaac ggctccaaga agaacaatgg caaaaccttt
      721 taggcggccg cttaattaat tgatccgggt tattagtaca tttattaagc gctagattct
      781 gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa
      841 tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat
      901 ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt
      961 tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaact
//
