LOCUS       sequence-83273-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Artificial transcription factor with VP16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Senecal A, Munsky B, Proux F, Ly N, Braye FE, Zimmer C, Mueller F, 
            Darzacq X
  TITLE     Transcription Factors Modulate c-Fos Transcriptional Bursts.
  JOURNAL   Cell Rep. 2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053.
  PUBMED    24981864
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053."
COMMENT     Sequence Label: pAF1-VP16
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..29
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(44..49,50..67,68..74)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    82..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     87..109
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     106..122
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     106..122
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             118..525
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     141..161
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        143..161
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(208..227)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(209..227)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(237..254)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(237..253)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(246..268)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
       61 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      121 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggaggacc
      181 tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
      241 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      301 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      361 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcccgtctc
      421 caggggggca cccgtcagtg cattgcgcga tgcatgcact gcctccattg cggtcacgcc
      481 gccacgtttt gcaatcttca caagttggcc tgtgtccaac tgtaacggcg gacctctcaa
      541 ctcccccgca tccgtgagca aggcctccag ggcgcgtgcg ccggaccact gtttgccgac
      601 gccaacgatg tcttcgtgtg tcgcctctgg caacgccgtg attatgtgct gatacgtgac
      661 agcgacggtc cctaacgctg ccgggtgttg gctgagcgca acgatgtgcg cgtgtgtaaa
      721 cccatggccc accagtgcct cgtggtgctg cgccactgtc gaacgcacct tcggtttgat
      781 cttctcttgc tgctgctgac tgtagccgag cgtgcgtaga tccacctgcg cggccggcga
      841 aa
//
