LOCUS       sequence-83280-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of constitutively active Ste7 kinase in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Mok J, Kim PM, Lam HY, Piccirillo S, Zhou X, Jeschke GR, Sheridan 
            DL, Parker SA, Desai V, Jwa M, Cameroni E, Niu H, Good M, Remenyi A,
            Ma JL, Sheu YJ, Sassi HE, Sopko R, Chan CS, De Virgilio C, 
            Hollingsworth NM, Lim WA, Stern DF, Stillman B, Andrews BJ, Gerstein
            MB, Snyder M, Turk BE
  TITLE     Deciphering protein kinase specificity through large-scale analysis 
            of yeast phosphorylation site motifs.
  JOURNAL   Sci Signal. 2010 Feb 16;3(109):ra12. doi: 10.1126/scisignal.2000482.
  PUBMED    20159853
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2010-02-16"; volume: "3(109):ra12. doi"; pages: " 
            10.1126/scisignal.2000482"
COMMENT     Sequence Label: pGEX4T2-Ste7-EE
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(241..270)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aacgcgcgag gcagatcgtc agtcagtcac gatgcggccg caagcttctc tcaatcgtga
       61 aattttctgt acataaagtg ctactttgat gattattttt aaaaatattg ttacattaga
      121 aaattggttt gtgatttaca taatcaggag agtctatata tatatgaata tagctttgag
      181 aaagggatgt gaaaaaaagt gcaatatgtt cctaactaat gttatcgcat gcattcaatt
      241 caagtcctct tcagaaatga gcttttgctc atgggttgat ctttccgatt gtttcttttc
      301 taacttggca cggtccaagg cttctctttt aattctcttg tcttccttta ttttagattt
      361 tatttttcta caccaatgtc taaatttatc atctttagac ggtgatacgt atttcattat
      421 aagatcatga tgtagcaatt catgaatcga tgacctttcc ctttcattct taatacaaca
      481 cctattgaca aaatctgtca tttccttgga atagatacgg tctttgggta atcttggtga
      541 aggctcgttg acaatacgtt gcagcaaatc caatatgcca tcaggtgtat cgttatgccc
      601 acctagggga aactctccag ttaccagctc gatgatcatt aagcccaatg accaaacgtc
      661 ccctttgata gaataaacgt ttccttgtat cctctctggt gacatataag tggacgttcc
      721 aacaaattca tcagcgatct catttatgag ttttttggaa actccaaaat cacataactt
      781 aatctgcccc ttactattaa tcagaacatt ggaaggcttg atatcacgat gaatgatctt
      841 atattgtcgg tacaaatgat ccaagccatt tagtacgcca tacgctattt ttgatattgt
      901 taattcgttg aaccaggttt tc
//
