LOCUS       sequence-83281-e        8913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains crtE, crtB, crtI, and idi genes of Erwinia herbicola 
            (Pantoea agglomerans) Eho10, and thereby produces lycopene in 
            Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Cunningham FX Jr, Lee H, Gantt E
  TITLE     Carotenoid biosynthesis in the primitive red alga Cyanidioschyzon 
            merolae.
  JOURNAL   Eukaryot Cell. 2007 Mar;6(3):533-45. Epub 2006 Nov 3.
  PUBMED    17085635
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell."; date: "2007-03"; volume: "6(3)"; pages: "533-45. Epub 2006 
            Nov 3"
COMMENT     Sequence Label: pAC-LYCipi
FEATURES             Location/Qualifiers
     source          1..8913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1505..1510,1511..1527,1528..1533)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8249..8268)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(join(8473..8913,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
     1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
     1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
     1861 ccgtcctgtg gatctaaagg cacagcgtct catgcttcgc acaatgtaaa actgcttcag
     1921 aacctggcga gagctatccg cgcggtctac ggttaactga tactaaaaga caattcagcg
     1981 ggtaaccttg caatggtgag tggcagtaaa gcgggcgttt cgcctcatcg cgaaatagaa
     2041 gtaatgagac aatccattga cgatcacctg gctggcctgt tacctgaaac cgacagccag
     2101 gatatcgtca gccttgcgat gcgtgaaggc gtcatggcac ccggtaaacg gatccgtccg
     2161 ctgctgatgc tgctggccgc ccgcgacctc cgctaccagg gcagtatgcc tacgctgctc
     2221 gatctcgcct gcgccgttga actgacccat accgcgtcgc tgatgctcga cgacatgccc
     2281 tgcatggaca acgccgagct gcgccgcggt cagcccacta cccacaaaaa atttggtgag
     2341 agcgtggcga tccttgcctc cgttgggctg ctctctaaag cctttggtct gatcgccgcc
     2401 accggcgatc tgccggggga gaggcgtgcc caggcggtca acgagctctc taccgccgtg
     2461 ggcgtgcagg gcctggtact ggggcagttt cgcgatctta acgatgccgc cctcgaccgt
     2521 acccctgacg ctatcctcag caccaaccac ctcaagaccg gcattctgtt cagcgcgatg
     2581 ctgcagatcg tcgccattgc ttccgcctcg tcgccgagca cgcgagagac gctgcacgcc
     2641 ttcgccctcg acttcggcca ggcgtttcaa ctgctggacg atctgcgtga cgatcacccg
     2701 gaaaccggta aagatcgcaa taaggacgcg ggaaaatcga cgctggtcaa ccggctgggc
     2761 gcagacgcgg cccggcaaaa gctgcgcgag catattgatt ccgccgacaa acacctcact
     2821 tttgcctgtc cgcagggcgg cgccatccga cagtttatgc atctgtggtt tggccatcac
     2881 cttgccgact ggtcaccggt catgaaaatc gcctgatacc gcccttttgg gttcaagcag
     2941 tacataacga tggaaccaca ttacaggagt agtgatgaat gaaggacgag cgccttgttc
     3001 agcgtaagaa cgatcatctg gatatcgttc tcgacccccg tcgcgccgta actcaggcta
     3061 gcgcaggttt tgagcgctgg cgctttaccc actgcgccct gccagagctg aattttagcg
     3121 acatcacgct ggaaaccacc ttcctgaatc ggcagctaca ggctccgctg ctgatcagct
     3181 ccatgaccgg cggcgttgag cgctcgcgcc atatcaaccg ccacctcgcc gaggcggcgc
     3241 aggtgctaaa aattgcgatg ggggtgggct cccagcgcgt cgccattgag agcgacgcgg
     3301 gcttagggct ggataaaacc ctgcggcagc tggctccgga cgtgccgctg ctggcgaacc
     3361 tcggcgcggc gcagctgacc ggcagaaaag gtattgatta cgcccgacgg gccgtggaga
     3421 tgatcgaggc ggatgcgctg attgtgcacc taaacccgct gcaggaggcg ctacagcccg
     3481 gcggcgatcg cgactggcgc ggacggctgg cggctattga aactctggtc cgcgagctgc
     3541 ccgttccgct ggtggtgaaa gaggtgggag ccggtatctc ccgaaccgtg gccgggcagc
     3601 tgatcgatgc cggcgttacc gtgattgacg tcgcgggcgc gggcggcacc agctgggccg
     3661 ccgttgaagg cgagcgggcg gccaccgagc agcagcgcag cgtggccaac gtctttgccg
     3721 actgggggat ccccaccgct gaggcgctgg ttgacattgc cgaggcctgg ccgcagatgc
     3781 cccttattgc ctcgggcggg attaaaaacg gcgtcgacgc ggcgaaagcg ctgcggctcg
     3841 gcgcgtgcat ggtagggcag gccgccgccg tgctcggcag cgcaggcgtc tccacggaga
     3901 aggtgatcga tcacttcaac gtgattattg agcagctgcg ggtggcctgc ttctgcaccg
     3961 gcagccgcag cctgagcgat ctaaagcagg ctgatatccg ctatgtgcgg gatacgccat
     4021 gagccatttt gccattgtgg caccgccgct ctacagtcat gcggtggcgc tgcatgccct
     4081 ggcgctggag atggcccaac gcggccacat cgttctcgac ccccgtcgcg ccgtaactca
     4141 ggctagcgca ggttttgagc gctggcgctt tacccactgc gccctgccag agctgaattt
     4201 tagcgacatc acgctggaaa ccaccttcct gaatcggcag ctacaggctc cgctgctgat
     4261 cagctccatg accggcggcg ttgagcgctc gcgccatatc aaccgccacc tcgccgaggc
     4321 ggcgcaggtg ctaaaaattg cgatgggggt gggctcccag cgcgtcgcca ttgagagcga
     4381 cgcgggctta gggctggata aaaccctgcg gcagctggct ccggacgtgc cgctgctggc
     4441 gaacctcggc gcggcgcagc tgaccggcag aaaaggtatt gattacgccc gacgggccgt
     4501 ggagatgatc gaggcggatg cgctgattgt gcacctaaac ccgctgcagg aggcgctaca
     4561 gcccggcggc gatcgcgact ggcgcggacg gctggcggct attgaaactc tggtccgcga
     4621 gctgcccgtt ccgctggtgg tgaaagaggt gggagccggt atctcccgaa ccgtggccgg
     4681 gcagctgatc gatgccggcg ttaccgtgat tgacgtcgcg ggcgcgggcg gcaccagctg
     4741 ggccgccgtt gaaggcgagc gggcggccac cgagcagcag cgcagcgtgg ccaacgtctt
     4801 tgccgactgg gggatcccca ccgctgaggc gctggttgac attgccgagg cctggccgca
     4861 gatgcccctt attgcctcgg gaatgcgggc tgggctattt caccctacca ctggctattc
     4921 gctgccgctg gcggtggccc ttgccgacgc gattgccgac agcccgcggc tgggcagcgt
     4981 tccgctctat cagctcaccc ggcagtttgc cgaacgccac tggcgcaggc agggattctt
     5041 ccgcctgctg aaccggatgc ttttcctggc cgggcgcgag gagaaccgct ggcgggtgat
     5101 gcagcgcttt tatgggctgc cggagcccac cgtagagcgc ttttacgccg gtcggctctc
     5161 tctctttgat aaggcccgca ttttgacggg caagccaccg gttccgctgg gcgaagcctg
     5221 gcgggcggcg ctgaaccatt ttcctgacag acgagataaa ggatgaaaaa aaccgttgtg
     5281 attggcgcag gctttggtgg cctggcgctg gcgattcgcc tgcaggcggc agggatccca
     5341 accgtactgc tggagcagcg ggacaagccc ggcggtcggg cctacgtctg gcatgaccag
     5401 ggctttacct ttgacgccgg gccgacggtg atcaccgatc ctaccgcgct tgaggcgctg
     5461 ttcaccctgg ccggcaggcg catggaggat tacgtcaggc tgctgccggt aaaacccttc
     5521 taccgactct gctgggagtc cgggaagacc ctcgactatg ctaacgacag cgccgagctt
     5581 gaggcgcaga ttacccagtt caacccccgc gacgtcgagg gctaccggcg ctttctggct
     5641 tactcccagg cggtattcca ggagggatat ttgcgcctcg gcagcgtgcc gttcctctct
     5701 tttcgcgaca tgctgcgcgc cgggccgcag ctgcttaagc tccaggcgtg gcagagcgtc
     5761 taccagtcgg tttcgcgctt tattgaggat gagcatctgc ggcaggcctt ctcgttccac
     5821 tccctgctgg taggcggcaa ccccttcacc acctcgtcca tctacaccct gatccacgcc
     5881 cttgagcggg agtggggggt ctggttccct gagggcggca ccggggcgct ggtgaacggc
     5941 atggtgaagc tgtttaccga tctgggcggg gagatcgaac tcaacgcccg ggtcgaagag
     6001 ctggtggtgg ccgataaccg cgtaagccag gtccggctgg cggatggtcg gatctttgac
     6061 accgacgccg tagcctcgaa cgctgacgtg gtgaacacct ataaaaagct gctcggccac
     6121 catccggtgg ggcagaagcg ggcggcagcg ctggagcgca agagcatgag caactcgctg
     6181 tttgtgctct acttcggcct gaaccagcct cattcccagc tggcgcacca taccatctgt
     6241 tttggtcccc gctaccggga gctgatcgac gagatcttta ccggcagcgc gctggcggat
     6301 gacttctcgc tctacctgca ctcgccctgc gtgaccgatc cctcgctcgc gcctcccggc
     6361 tgcgccagct tctacgtgct ggccccggtg ccgcatcttg gcaacgcgcc gctggactgg
     6421 gcgcaggagg ggccgaagct gcgcgaccgc atctttgact accttgaaga gcgctatatg
     6481 cccggcctgc gtagccagct ggtgacccag cggatcttta ccccggcaga cttccacgac
     6541 acgctggatg cgcatctggg atcggccttc tccatcgagc cgctgctgac ccaaagcgcc
     6601 tggttccgcc cgcacaaccg cgacagcgac attgccaacc tctacctggt gggcgcaggt
     6661 actcaccctg gggcgggcat tcctggcgta gtggcctcgg cgaaagccac cgccagcctg
     6721 atgattgagg atctgcaatg agccaaccgc cgctgcttga ccacgccacg cagaccatgg
     6781 ccaacggctc gaaaagtttt gccaccgctg cgaagctgtt cgacccggcc acccgccgta
     6841 gcgtgctgat gctctacacc tggtgccgcc actgcgatga cgtcattgac gaccagaccc
     6901 acggcttcgc cagcgaggcc gcggcggagg aggaggccac ccagcgcctg gcccggctgc
     6961 gcacgctgac cctggcggcg tttgaagggg ccgagatgca ggatccggcc ttcgctgcct
     7021 ttcaggaggt ggcgctgacc cacggtatta cgccccgcat ggcgctcgat cacctcgacg
     7081 gctttgcgat ggacgtggct cagacccgct atgtcacctt tgaggatacg ctgcgctact
     7141 gctatcacgt ggcgggcgtg gtgggtctga tgatggccag ggtgatgggc gtgcgggatg
     7201 agcgggtgct ggatcgcgcc tgcgatctgg ggctggcctt ccagctgacg aatatcgccc
     7261 gggatattat tgacgatgcg gctattgacc gctgctatct gcccgccgag tggctgcagg
     7321 atgccgggct gaccccggag aactatgccg cgcgggagaa tcgggccgcg ctggcgcggg
     7381 tggcggagcg gcttattgat gccgcagagc cgtactacat ctcctcccag gccgggctac
     7441 acgatctgcc gccgcgctgc gcctgggcga tcgccaccgc ccgcagcgtc taccgggaga
     7501 tcggtattaa ggtaaaagcg gcgggaggca gcgcctggga tcgccgccag cacaccagca
     7561 aaggtgaaaa aattgccatg ctgatggcgg caccggggca ggttattcgg gcgaagacga
     7621 cgagggtgac gccgcgtccg gccggtcttt ggcagcgtcc cgtttaggcg ggcggccatg
     7681 acgttcacgc aggatcgcct gtaggtcggc aggcttgcgg gcgtaaataa aaccgaagga
     7741 gacgcagccc tcccggccgc gcaccgcgtg gtgcaggcgg tgggcgacgt agagccgctt
     7801 caggtagccc cggcgcggga tcccgcctcg ccgtgctgtc cggtctcaac ctgatccgcc
     7861 agaatcgagc caacgggatc ggccagcacg aattcggtat gcggagagtg tttggcaaac
     7921 catgcctgca agccacccag cgtgccgccg gagccaacgc ctactaccac ggcatcaacc
     7981 ctgcctgcca gctggtcgaa cagctccggt gcggtggtgg tggcgtgcgc cagcgggttg
     8041 gccgggttag agaactgatc aatatagtaa gcacccggcg tctcctctgc caggcggcgg
     8101 gcgtagtcct gatagtactc cgggtggccc tttgtcacgt cggagcgggt caggcgcaca
     8161 tcaacaccca gcgcacgcag gtggtagatc ctctacgccg gacgcatcgt ggccggcatc
     8221 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     8281 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     8341 gtggccgggg gactgttggg cgccatctcc ttgtcgaatt tgctttcgaa tttctgccat
     8401 tcatccgctt attatcactt attcaggcgt agcaccaggc gtttaagggc accaataact
     8461 gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     8521 cattctgccg acatggaagc catcacagac ggcatgatga acctgaatcg ccagcggcat
     8581 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     8641 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     8701 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     8761 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     8821 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     8881 tatcaccagc tcaccgtctt tcattgccat acg
//
