LOCUS       sequence-83283-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pBID-UASC-VG
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..233)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          363..428
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             558..578
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
      121 atatgttgtg ttttacagta ttatgtantc tgttttttat gcaaaatcta atttaatata
      181 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatatcg
      241 agctctcccg ggaattcgga tccggccctc gagggtacct ctagaggatc tttgtgaagg
      301 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
      361 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
      421 tattttagat tccaacctat ggaactgatg aatgggagca gnggtggaat gcctttaatg
      481 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
      541 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
      601 canaattgct aagttttttg agtcatgctg tgtttagtaa ta
//
