LOCUS       sequence-83288-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral U6 driven sgRNA cloning vector where guide sequences are 
            inserted between BfuAI sites, improved cassette cloning efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     Improved U6-sgRNA vector for expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLKO.1-puro U6 sgRNA BfuAI large stuffer
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(1..76)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(289..308)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta
       61 tttctagctc taaaacgtca gcaggttctt gatccgtcgg aattgccagc tggggcgccc
      121 tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc
      181 tgatggcgca ggggatcaag atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
      241 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
      301 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
      361 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
      421 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
      481 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
      541 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
      601 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
      661 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
      721 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
      781 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
      841 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca g
//
