LOCUS       sequence-8330-ex       11158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Bhattacharyya D, Glick BS
  TITLE     Two mammalian Sec16 homologues have nonredundant functions in 
            endoplasmic reticulum (ER) export and transitional ER organization.
  JOURNAL   Mol Biol Cell. 2007 Mar;18(3):839-49. doi: 10.1091/mbc.e06-08-0707
  PUBMED    17192411
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1091/mbc"; 
            journalName: "Mol Biol Cell"; date: "2007-03"; volume: "18"; issue: 
            "3"; pages: "839-49"
COMMENT     Sequence Label: pmGFP-Sec16L
FEATURES             Location/Qualifiers
     source          1..11158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7946..8067
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7983..8002)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8037..8056
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8074..8529)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8211..8232)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8423..8442
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8556..8660
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8662..9019
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8662..8853
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8685..8705)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8870..9005
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9054..9848
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9054..9848
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9108..9127)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9718..9737
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10036..10055)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10080..10127
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10456..11044
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10945..10964
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagga atgcctgggc tcgaccgacc cctgagcagg
     1381 caaaacccac atgacggtgt ggtcacccca gcagcatccc cttccctccc tcagcctggt
     1441 ctgcagatgc caggacagtg ggggccagtg cagggaggcc cacagccctc ggggcaacat
     1501 cgttcaccct gccctgaagg acctgttccc agcggggtgc cctgtgccac cagcgttcct
     1561 catttcccca ccccgtccat cctacatcag ggccctggtc atgagcaaca cagccctctg
     1621 gtggctcccc cagcagcctt gcccagtgac ggaagagacg aggtgagcca cttgcaaagt
     1681 ggaagccacc tggccaataa ctctgatcct gaaagtacat tcaggcaaaa tcccagaatt
     1741 gtgaatcact gggcaagccc agagctcagg cagaatccag gagtgaagaa tgagcaccgg
     1801 cccgcctctg ctcttgtgaa ccccctcgcc cggggagata gcccagaaaa ccgtacgcac
     1861 cacccactgg gggctggggc cgggtctggc tgtgccccgc tagaagcaga ctcaggagct
     1921 tcaggagctc tggcgatgtt tttccaaggg ggagagacag aaaatgagga gaatctctca
     1981 tctgaaaaag caggcttatc tggtcaagcg gactttgacg atttctgctc cagccctggg
     2041 ctaggccgtc cgcccgcacc tacacacgtg ggggcaggca gcctctgcca ggcccttctc
     2101 ccaggcccca gcaatgaggc tgctggtgat gtgtggggtg acacagcgag cacaggggtg
     2161 ccggatgcca gcggctcgca gtatgagaat gttgagaact tagaatttgt tcagaatcaa
     2221 gaagttctgc caagtgagcc cctcaatttg gacccttcct ccccgagtga ccagttcaga
     2281 tatgggcccc ttcctgggcc agctgtgccc aggcatggtg ctgtgtgcca caccggagcc
     2341 cctgatgcca cactgcatac agtgcaccct gacagcgtgt catccagcta tagcagcaga
     2401 agccacggaa ggctctcagg ctcagccagg ccccaggagc tggttggcac attcattcag
     2461 caagaagttg gaaaacccga ggatgaagct tcaggtagtt tttttaagca aatcgattct
     2521 tctcccgtag gaggtgaaac agacgagacc actgtgagcc agaattaccg tggcagcgtg
     2581 tcccagccct caaccccgag ccccccgaaa cctacaggaa tatttcagac aagtgcaaat
     2641 agttctttcg aaccggtaaa atctcactta gttggggtaa aaccatttga ggcagatcgc
     2701 gccaacgtgg ttggtgaagt aagggagacc tgtgtccgcc agaagcagtg cagaccagct
     2761 gccgccctgc ccgatgcttc ccctggcaac ctggagcagc caccagacaa catggagacc
     2821 ctctgtgcac cccaggtctg tcccctgcct cttaactcca ccacggaagc tgtgcacatg
     2881 cttccgcacg caggggcacc gcccttggat actgtgtatc cagcacccga gaagaggcct
     2941 tcagccagga cccaggggcc cgtgaagtgt gagagcccag caacgactct gtgggcgcaa
     3001 agtgagctgc cagattttgg aggcaacgtc cttctggccc ctgcagcccc ggcgctttat
     3061 gtgtgtgcaa aacctcagcc acctgttgtt cagcctccag aagaggcgat gtccgggcag
     3121 cagtcacgga acccaagctc ggcggccccg gtgcagagcc gaggtggcat tggtgcttct
     3181 gagaaccttg agaatcctcc caaaatggga gaggaggagg cccttcagtc ccaggcgagt
     3241 tctggttatg caagtttatt atcctcaccg cccactgagt ctctgcagaa tcctccagtc
     3301 ttgattgctc agcctgatca cagctataat ctggctcagc ccattaactt ttctgtgtcc
     3361 ttatcgaact ctcatgagaa gaatcagtcc tggagagagg ctttggtggg ggatagacct
     3421 gcagtcagca gttgggctct cggtggtgat tctggggaga acacttcttt gtctgggatt
     3481 ccaaccagct ctgtccttag cttgtctctg cctagcagtg ttgcccaaag taattttcca
     3541 caaggttctg gtgcttccga aatggtttct aatcagcctg ctaatttgct ggttcaacca
     3601 ccatcccagc cagttccaga gaacttggtt ccagaaagtc aaaaggatcg taaggcagga
     3661 agtgctcttc ccggatttgc taatagccct gctggaagca caagtgtggt gttagttcca
     3721 cctgcacacg gcaccctggt gcctgatggt aataaggcaa accattccag tcatcaggaa
     3781 gacacttacg gagccctaga ctttacctta agcaggactt tggaaaatcc tgtaaacgtg
     3841 tacaacccgt cccattctga cagcctcgct tctcagcaaa gtgttgccag tcatcccaga
     3901 caatctgggc ctggggcgcc taaccttgac cgtttttatc agcaggtcac gaaagatgcc
     3961 cagggccagc ctggcctcga aagagcccag caggagctgg tgccacccca gcaacaggct
     4021 tctcccccac aactacccaa agccatgttt tcggagctgt caaatccaga aagtctgccc
     4081 gcacagggac aggcccagaa ctcagcacag tcaccagcaa gtctggttct ggtcgacgcg
     4141 ggtcagcagc tgccccctcg gcctcctcag tcctctagcg tgtctctggt gtccagtggc
     4201 tccggccagg cagctgtgcc gtcagagcag ccgtggccac agccagtgcc tgcacttgcc
     4261 cccggcccac cgcctcagga cctggccgcc tactactact accggccttt gtacgatgcc
     4321 taccagcctc agtactcttt gccgtaccca ccggagcctg gcgcagcctc cctctattac
     4381 caggatgtct acagcctcta tgagcctcga tacaggccct atgatggtgc tgcgtctgct
     4441 tacgcccaga actaccgcta tcccgagccc gagcggccca gctcccgagc cagccactcc
     4501 tcggaacggc cacctcccag gcaaggatat cctgaaggat actatagttc caaaagtgga
     4561 tggagcagtc agagcgatta ctatgcaagc tattactcca gccagtacga ttatggagat
     4621 ccaggtcact gggatcgtta ccactacagt gctagagtca gggacccccg cacctatgac
     4681 cggaggtatt ggtgtgatgc agagtatgac gcatacagga gagagcactc tgccttcggg
     4741 gacaggcccg agaaacgtga caacaactgg aggtacgatc ctcgcttcac ggggagtttt
     4801 gacgatgacc ccgatccgca cagagaccct tatggggaag aggtggaccg gcgcagcgtc
     4861 cacagcgagc actcggcacg gagcctgcac agcgcacaca gcctggccag ccgccgcagc
     4921 agcctcagct cccactcgca ccagagtcag atttacagaa gccacaatgt ggctgccggt
     4981 tcctacgagg ccccgcttcc tccaggctcc tttcacggcg attttgccta cggcacctac
     5041 cgcagcaatt tcagcagtgg ccccggcttc ccagagtatg gctaccctgc cgacaccgtc
     5101 tggcctgcca tggagcaagt ttcatcaaga ccaacttctc ctgaaaaatt ttcagtgcct
     5161 catgtctgtg ccaggtttgg ccctggcggt cagcttatca aagtgattcc caatctgcct
     5221 tcagaaggac agccggcctt ggtggaggtc cacagcatgg aggccttgct gcagcacacg
     5281 tctgagcagg aggagatgcg ggcgttcccg ggacccctgg ccaaagacga cacccataag
     5341 gtggatgtca ttaattttgc acagaacaaa gctatgaaat gtttgcagaa tgaaaactta
     5401 attgacaaag agtctgcaag tcttctttgg aattttattg ttctcttatg cagacaaaat
     5461 gggaccgtgg tagggaccga cattgcggag cttctgttac gagaccacag aacagtgtgg
     5521 cttcctggga agtcgcccaa tgaagcaaac ctgattgatt tcacgaatga ggcagtggag
     5581 caggtggaag aggaggagtc tggtgaggcc cagctctctt tcctcactgg tggtccggcg
     5641 gctgccgcca gctcgctcga gagagagacc gagaggttca gggagctgtt gctgtatggc
     5701 cgtaagaagg atgctttgga gtctgcaatg aagaatggcc tgtggggtca cgctctgcta
     5761 cttgcaagta agatggacag ccggacacac gcccgagtca tgaccaggtt tgctaacagc
     5821 ctcccaatca acgaccctct gcagacagtc taccagctca tgtccggacg gatgcctgcc
     5881 gcgtccacgt gctgtggaga cgagaaatgg ggagattgga ggccgcacct cgccatggtc
     5941 ttgtccaact tgaacaacaa catggacgtc gagtccagga cgatggctac catgggcgac
     6001 actctggctt caaggggcct cttggatgcg gcccacttct gctacctcat ggcccaggcg
     6061 ggatttggtg tttacacgaa gaaaactaca aagcttgtct taatcggatc caatcacagt
     6121 ttgccattct taaagttcgc aaccaacgaa gcaatccaga ggacggaagc ctatgagtac
     6181 gcccagtccc tgggtgccga gacctgcccc ctgcctagtt tccaggtgtt taagttcatc
     6241 tactcctgcc gcctggcgga aatggggctg gccacgcaag ccttccacta ctgtgaggcc
     6301 atcgcgaaga gcatcctgac gcagccgcac ctgtattccc cggtgttgat cagccagctt
     6361 gtgcagatgg cttcccagtt acgactcttc gatccccagc tgaaagagaa gccagaagag
     6421 gagtccttgg ccgcacccac gtggctggtt cacctgcagc aggtggagcg gcagattaag
     6481 gagggggctg gagtatggca tcaggatgga gccctcccgc agcagtgtcc tggcactccg
     6541 agttccgaga tggagcagtt ggacaggcca ggactcagtc agccaggagc cctggggatc
     6601 gccaaccctc tgctggcggt gcctgcaccg agccctgagc actcgagccc gagcgtgcgg
     6661 ctgctgccct cagctccgca gacgctccct gacggcccat tggccagtcc tgccagagtg
     6721 ccgatgttcc cagtgccact gcccccgggg cccctggagc cgggtcctgg ctgtgtgacc
     6781 ccagggcctg cacttggctt cctggagccc tccgggcctg gcctcccacc tggtgtgcca
     6841 cctctgcagg aaaggagaca cttgctccag gaagccagga gcccagaccc agggatagtg
     6901 ccgcaggagg cgcctgttgg aaactcactt tccgagctaa gcgaagaaaa ttttgatgga
     6961 aaatttgcta atctgacccc ctcgaggacg gtgccagact cggaggcccc cccagggtgg
     7021 gatcgtgccg actcgggtcc cacgcagcca cctctgtctc tctcacccgc tcccgaaaca
     7081 aagagacccg gacaggcagc caagaaagaa acgaaggaac ctaagaaggg tgaatcctgg
     7141 ttctttcgtt ggctacctgg aaagaaaaag acagaagctt atttgccaga tgacaagaac
     7201 aaatcgattg tttgggatga aaagaaaaac cagtgggtga atttaaatga gccagaagag
     7261 gagaagaaag ccccgccccc acctccaacc tcgatgccca agactgtgca agctgccccg
     7321 cctgccctcc cagggcctcc tggagccccc gtgaacatgt actctagaag agcagcagga
     7381 accagagctc gctacgttga cgtcctgaac ccaagcggga cccagcggag cgagccggct
     7441 ctcgctcctg cggactttgt cgctccactc gcgccactcc caattccttc taacttgttc
     7501 gtgccaaccc cagatgcaga agaaccacag cttccagacg ggactggcag ggaagggcct
     7561 gcagcagcta ggggcctggc caatccagag cctgccccag agcccaagct ttcgcgctgt
     7621 agttcaatga gttcattatc acgtgaagtg agccagcatt ttaatcaggc tcctggcgac
     7681 ctccctgctg cagggggccc tcccagcggg gccatgccct tctacaaccc tgctcagctg
     7741 gcacaggcct gcgccacctc cgggagctca aggctaggga ggattggcca gaggaagcac
     7801 ctggtgctga actaggggat ccaccggatc tagataactg atcataatca gccataccac
     7861 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     7921 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     7981 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8041 tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa
     8101 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     8161 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     8221 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     8281 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     8341 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     8401 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     8461 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     8521 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8581 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8641 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     8701 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8761 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8821 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     8881 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8941 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9001 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     9061 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     9121 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     9181 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     9241 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     9301 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     9361 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     9421 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     9481 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     9541 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     9601 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     9661 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     9721 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     9781 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     9841 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     9901 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     9961 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    10021 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
    10081 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
    10141 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
    10201 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
    10261 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
    10321 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    10381 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    10441 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10501 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10561 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
    10621 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10681 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10741 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10801 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10861 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10921 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10981 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    11041 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    11101 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
