LOCUS       sequence-83303-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid template for PCR of cassettes for HR, used to introduce IFP 
            F[1] fragments 3' to the ORF of the genes to study. Plasmid confers 
            ampicillin resistance to DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: pAG25-L-IFP-F[1] (clon 3)
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      568..765
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tcttgacgac acggcttacc ggtaccgcac cagtgtcccg ggggacgccg aggccatcga
       61 ggcactggat gggtccttca ccaccgacac cgtcttccgc gtcaccgcca ccggggacgg
      121 cttcaccctg cgggaggtgc cggtggaccc gcccctgacc aaggtgttcc ccgacgacga
      181 atcggacgac gaatcggacg acggggagga cggcgacccg gactcccgga cgttcgtcgc
      241 gtacggggac gacggcgacc tggcgggctt cgtggtcgtc tcgtactccg gctggaaccg
      301 ccggctgacc gtcgaggaca tcgaggtcgc cccggagcac cgggggcacg gggtcgggcg
      361 cgcgttgatg gggctcgcga cggagttcgc ccgcgagcgg ggcgccgggc acctctggct
      421 ggaggtcacc aacgtcaacg caccggcgat ccacgcgtac cggcggatgg ggttcaccct
      481 ctgcggcctg gacaccgccc tgtacgacgg caccgcctcg gacggcgagc aggcgctcta
      541 catgagcatg ccctgcccct aatcagtact gacaataaaa agattcttgt tttcaagaac
      601 ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg
      661 atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa
      721 gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac
      781 taacgccgcc atccagtgtc gaaaacga
//
