LOCUS       sequence-83304-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid template for PCR of cassettes for HR, used to introduce IFP 
            F[2] fragments 3' to the ORF of the genes to study. Plasmid confers 
            ampicillin resistance to DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: pAG32-L-IFP-F[2] (clon 3)
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
       61 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
      121 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
      181 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
      241 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
      301 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
      361 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
      421 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
      481 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
      541 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
      601 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
      661 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
      721 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
      781 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
      841 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
      901 gactgtcggg cgtacaca
//
