LOCUS       sequence-83306-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] 
            (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid 
            confers ampicillin resistance to DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: pLpC-L-IFP-F[2] (mammalian)
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccttaa ttaattcgaa atcgatggtg
      121 gcggtggctc tggaggtggt gggtcctccg gagctcggga cagcattggc cctcacgccc
      181 tgaggaacgc catgtttgct ctggaaagcg ctccaaacct gcgggctctg gccgaagtcg
      241 caacacaaac tgtgagagaa ctgactggct tcgatcgggt gatgctgtac aaatttgccc
      301 ctgacgccac tggagagatg attgctgagg ccagacggga gggcatgcag gcttttctgg
      361 gccacaggtt tcccgcatcc cacacccctg cacaagctag ggccctctac acaagacacc
      421 tgctccggct gaccgcagac accagggctg cagcagtgcc cctcgacccc gtgctgaatc
      481 cccagacaaa tgctcctaca cctctgggcg gagctgtcct cagagctaca tccccaatgc
      541 acatgcagta cctgaggaat atgggagtgg gctcctccct gagcgtcagc gtcgtggtcg
      601 gcggccagct gtggggactg attgtctgcc accatcagac accctacgtg ctgccaccag
      661 atctgcggac caccctggag gagctgggga gaaagctgtc cgggcaggtg cagaggaaag
      721 aagcctaaga attctgcaga tatccatcac actggcggcc gctcgagtaa cgttactggc
      781 cgaagccgct tggaataaag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
      841 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
      901 taggggtctt
//
