LOCUS       sequence-83307-e         499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] 
            (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is 
            cloned in between PmeI/SalI.Confers ampicillin resistance to 
            DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: pLpC-Reg-L-IFP-F[2]+Venus (mammalian)
FEATURES             Location/Qualifiers
     source          1..499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactttcta tagggagacc
       61 caagcttggt accgagctcg gatccttaat taaaccatga gcatcgagat ccccgcgggg
      121 ctcacggagc tgctgcaggg cttcacggtg gaggtgctga ggcaccagcc cgccgacctg
      181 ctggagttcg cgctgcagca cttcacgcgg ctgcagcagg agaacgagcg caagggcgcc
      241 gcgcgcttcg gccatgaggg caggacctgg ggggacgcgg gcgcagccgc cgggggcgga
      301 acccccagta agggtgtcaa cttcgcggag gagcccatgc gctccgattc cgagaacggc
      361 gaagaggagg aggccgcgga agcaggggcg ttcaacgctc cagttataaa ccggttcaca
      421 aggcgtgcct cggtatgtgc agaagcttat aatcctgatg aagaagaaga tgatgcagag
      481 tccaggataa tacatccca
//
