LOCUS       sequence-83308-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary retroviral pl. (puromycin). Cat(PacI/ClaI):Linker 
            (GGGGS)X2+IFP-F[2]. In the second MCS, the Plum fluorescent protein 
            is cloned in between PmeI/SalI.Confers ampicillin resistance to 
            DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: pLpC-Cat-L-IFP-F[1]+Plum (mammalian)
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccttaat taaaccatgg gcaacgccgc cgccgccaag
      121 aagggcagcg agcaggagag cgtgaaagag ttcctagcca aagccaagga agatttcctg
      181 aaaaaatggg agaccccttc tcagaataca gcccagttgg atcagtttga tagaatcaag
      241 acccttggca ccggctcctt tgggcgagtg atgctggtga agcacaagga gagtgggaac
      301 cactacgcca tgaagatctt agacaagcag aaggtggtga agctaaagca gatcgagcac
      361 actctgaatg agaagcgcat cctgcaggcc gtcaacttcc cgttcctggt caaacttgaa
      421 ttctccttca aggacaactc aaacctgtac atggtcatgg agtatgtagc tggtggcgag
      481 atgttctccc acctacggcg gattggaagg ttcagcgagc cccatgcccg tttctacgcg
      541 gcgcagatcg tcctgacctt tgagtatctg cactccctgg acctcatcta ccgggacctg
      601 aagcccgaga atcttctcat cgaccagcag ggctatattc aggtgacaga cttcggtttt
      661 gccaagcgtg tgaaaggccg tacttggacc ttgtgtggga cccctgagta cttggccccc
      721 gagattatcc tgagcaaagg ctacaacaag gctgtggact ggtgggctct cggagtcctc
      781 atctacgaga tggctgctgg ttacccaccc ttcttcgctg accagcctat ccagatctat
      841 gagaaaatcg tctctgggaa ggtgcggttc ccatcccact tcagctctga cttgaaggac
      901 ctgctgcgga accttctgca
//
