LOCUS       sequence-83309-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker 
            (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein
            is cloned in between PmeI/SalI.Confers ampicillin resistance to 
            DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             169..225
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccttaat taaaccatga acactgaagc cgccaggcgt
      121 tctcgtgcga gaaagttgca aagaatgaaa caacttgaag acaaggttga agaattgctt
      181 tcgaaaaatt atcacttgga aaatgaggtt gccagattaa agaaattagt tggcgaacgc
      241 atcgatggtg gcggtggctc tggaggtggt gggtcctccg gagctcggga cagcattggc
      301 cctcacgccc tgaggaacgc catgtttgct ctggaaagcg ctccaaacct gcgggctctg
      361 gccgaagtcg caacacaaac tgtgagagaa ctgactggct tcgatcgggt gatgctgtac
      421 aaatttgccc ctgacgccac tggagagatg attgctgagg ccagacggga gggcatgcag
      481 gcttttctgg gccacaggtt tcccgcatcc cacacccctg cacaagctag ggccctctac
      541 acaagacacc tgctccggct gaccgcagac accagggctg cagcagtgcc cctcgacccc
      601 gtgctgaatc cccagacaaa tgctcctaca cctctgggcg gagctgtcct cagagctaca
      661 tccccaatgc acatgcagta cctgaggaat atgggagtgg gctcctccct gagcgtcagc
      721 gtcgtggtcg gcggccagct gtggggactg attgtctgcc accatcagac accctacgtg
      781 ctgccaccag atctgcggac caccctggag gagctgggga gaaagctgtc cgggcaggtg
      841 cagaggaaag aagcctaaga attctgcaga tatccatcac actggcggcc gctcgagta
//
