LOCUS       sequence-8331-ex        6673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Wickersham IR, Lyon DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, 
            Young JA, Callaway EM
  TITLE     Monosynaptic restriction of transsynaptic tracing from single, 
            genetically targeted neurons.
  JOURNAL   Neuron. 2007 Mar 1. 53(5):639-47.
  PUBMED    17329205
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2007 Mar 1. 53(5):639-47."
COMMENT     Sequence Label: pCMMP-TVA800
FEATURES             Location/Qualifiers
     source          1..6673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        84..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    813..1170
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1235..1651
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1581..1603
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1619..1635
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     1973..1989
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(4054..4070)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4054..4070)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4067..4089)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4078..4094
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4102..4108,4109..4126,4127..4132))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4147..4168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4285..4302)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4456..5044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4536..4555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5215..6006,6007..6075))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5838..5857
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6076..6180)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6248..6266
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6304..6326)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6426..6445
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6639..6661
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6653..6670
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6654..6670
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tattggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc cttgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggttgt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      601 aaccggcgcc agtcctccga tagactgcgt cgcccgggta cccgtgttct caataaaccc
      661 tcttgcagtt gcatccgact cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
      721 ttgactaccc gtcagcgggg tctttcattt ggaggttcca ccgagatttg gagacccctg
      781 cccagggacc accgaccccc ccgccgggag gtaagctggc cagcaactta tctgtgtctg
      841 tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac
      901 tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc cggccgcaac
      961 cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg agtcctaaaa
     1021 tcccgatcgt ttaggactct ttggtgcacc ccccttagag gagggatatg tggttctggt
     1081 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggga
     1141 ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac
     1201 tgtgtttctg tatttgtctg aaaatatggg cccgggctag actgttacca ctcccttaag
     1261 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1321 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1381 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1441 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1501 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1561 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1621 ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga tcttatatgg
     1681 ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta ctaacagccc
     1741 ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct ggagacctct
     1801 ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt accgagtcgg
     1861 cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct ggaaaggacc
     1921 ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg cagcttggat
     1981 acacgccgcc cacgtggtgg taccgacatg gcgcggctgc tgcccgcgct gctgctgctg
     2041 ctgctgcccg gtaacgtgac cggtaacggg tccggtaacg gttctttgtc ccgttgcccc
     2101 cccggtcagt tccgctgctc ggagccgccc ggtgcccacg gggagtgtta cccgcaggac
     2161 tggctgtgcg acggacaccc cgactgcgac gacgggcggg acgagtgggg ctgcgggacc
     2221 agcgcgaccc ccgcggtgcc cacggacaac ggcacagagg ctcccactgt ccctgctcct
     2281 ggacgtgctc tgccagccag gaatcacggc cgcatgtgga tgctgatcac tgcagggatc
     2341 ttttgctgtg agctggtgag atgggactga actgctctgc ctgctgctca ccacggaacc
     2401 tgactcaatt tggacatgag ctctcaaatc tggtttgctg ttacactcaa atgctcccat
     2461 tggaaataat gggtctggat ggtggcaccc aaaatagggc aggaccttcc ccagggctgt
     2521 gaaatcaccc ctcctaaagg tggtggaaat tccctcactc tttttgccta caaggactat
     2581 tactattatt aaagctgctt ctcatcaccg tggcctttaa cctaaaggtt tgatcctcca
     2641 tggaggtgca gtgccaggtc ctgttctacc accccctcag cagagctcac tgagcccaca
     2701 tcagatgtgc actccctgag cctccttttg ctggaggctt tgaatgttcg gtgtctcttg
     2761 ctttcagctt gaaatactga attcgtcgac ggatccggat tagtccaatt tgttaaagac
     2821 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
     2881 gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
     2941 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
     3001 aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga
     3061 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccgctcagg gccaagaaca
     3121 gttggaacag gagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     3181 tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac
     3241 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     3301 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     3361 agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc
     3421 gtgttctcaa taaaccctct tgcagttgca tccgactcgt ggtctcgctg ttccttggga
     3481 gggtctcctc tgagtgattg actacccgtc agcggggtct ttcagtttct cccacctaca
     3541 caggtctcac taacattcct gatgtgccgc agggactccg tcagcccggt ttttgtttat
     3601 aataaaatgc aagaacagtg ttcccttcaa gccagactac atcctgactc tcggctttat
     3661 aaaagaatgt tgaagggctc tgtggactat ctgccacacg actttttaag atttttatgc
     3721 ctcctggatg agggatttag tcaatctatc ctcgtctatt ttgctggctt ctccgtattt
     3781 taaatttcta gtttgcactc ccttcctgag agcacggcga ttgcagagta gttaatactc
     3841 tgagggcagg cttctgtgaa aaggttgcct gggctcagtg tgagattttg ccataaaaag
     3901 gggtcctgcc cctgtgtaca gacagatcgg aatctagagt gcatactcag agtccccgcg
     3961 gttccggggc tctgatctca gggcatcttt gcctagagat cctctacgcc ggacgcatcg
     4021 tggccgggta ccgagctcga attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4081 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4141 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4201 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4261 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4441 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4861 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     4921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5521 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5581 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5641 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5701 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5761 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5821 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5881 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5941 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6001 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6061 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6121 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6181 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     6241 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     6301 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6361 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     6421 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     6481 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     6541 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     6601 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     6661 gacggccagt gcc
//
