LOCUS       sequence-83311-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a mammalian scaffold protein that mediates the activation of a MAP 
            kinase signaling pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Whitmarsh AJ, Cavanagh J, Tournier C, Yasuda J, Davis RJ
  TITLE     A mammalian scaffold complex that selectively mediates MAP kinase 
            activation.
  JOURNAL   Science. 1998 Sep 11;281(5383):1671-4.
  PUBMED    9733513
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-09-11"; volume: "281(5383)"; pages: "1671-4"
COMMENT     Sequence Label: pcdna3 T7 Jip1b(471-707)(C478S, C667S and C699T)
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             85..117
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(872..890)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(873..890)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttatggct agcatgactg gtggacagca aatgggtgga
      121 tccgagtcct ttgggctgtt ctccagcgtc atcaatgggg aggagcatga gcaaacccat
      181 cgggctatat tcaggtttgt gcctcggcat gaagatgaac ttgagctgga agtggatgac
      241 cccctgctgg tggagctgca ggcagaagac tattggtatg aggcctataa catgcgcacc
      301 ggagcccgcg gggtcttccc tgcctactat gccattgagg tcaccaagga gcctgagcac
      361 atggcagccc ttgccaaaaa cagcgactgg attgaccagt tccgggtgaa gttcctgggg
      421 tctgtccagg ttccttatca caagggcaat gatgtcctct gtgctgctat gcaaaagatc
      481 gccaccaccc gccggctcac cgtgcacttt aacccgccct ccagctgtgt ccttgagatc
      541 agtgtcaggg gtgtcaagat aggcgtcaaa gctgatgatg ctctggaggc caagggaaat
      601 aaatgtagcc acttcttcca gctaaagaac atctctttct gtggatacca tccaaagaat
      661 aacaagtact ttgggtttat cactaagcac cctgctgacc accggtttgc cagccatgtc
      721 tttgtgtctg aagattccac caaagccctg gcggagtctg tggggcgtgc atttcagcag
      781 ttctacaagc agtttgtgga gtataccact cctacagaag atatctactt ggagtaggcg
      841 gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc
//
