LOCUS       sequence-83312-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Luo Y, Liu C, Cerbini T, San H, Lin Y, Chen G, Rao MS, Zou J
  TITLE     Stable Enhanced Green Fluorescent Protein Expression After 
            Differentiation and Transplantation of Reporter Human Induced 
            Pluripotent Stem Cells Generated by AAVS1 Transcription 
            Activator-Like Effector Nucleases.
  JOURNAL   Stem Cells Transl Med. 2014 May 15. pii: sctm.2013-0212.
  PUBMED    24833591
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Transl Med. 2014 May 15. pii: sctm.2013-0212."
COMMENT     Sequence Label: pZT-AAVS1-L1
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             140..205
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             212..232
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagctgatcc actagtccag
      121 tgtggtggaa ttcgccatgg actacaaaga ccatgacggt gattataaag atcatgacat
      181 cgattacaag gatgacgatg acaagatggc ccccaagaag aagaggaagg tgggcatcca
      241 cggggtacct atggtggact tgaggacact cggttattcg caacagcaac aggagaaaat
      301 caagcctaag gtcaggagca ccgtcgcgca acaccacgag gcgcttgtgg ggcatggctt
      361 cactcatgcg catattgtcg cgctttcaca gcaccctgcg gcgcttggga cggtggctgt
      421 caaataccaa gatatgattg cggccctgcc cgaagccacg cacgaggcaa ttgtaggggt
      481 cggtaaacag tggtcgggag cgcgagcact tgaggccttg ctgactgtgg cgggtgagct
      541 tagggggcct ccgctccagc tcgacaccgg gcagctgctg aagatcgcga agagaggggg
      601 agtaacagcg gtagaggcag tgcacgcctg gcgcaatgcg ctcaccgggg cccccctgaa
      661 cctgaccccg gaccaagtgg tggctatcgc cagccacgat ggcggcaagc aagcgctcga
      721 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgactc cggaccaagt
      781 ggtggctatc gccagccacg atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
      841 gccggtgctg tgccaggacc atggc
//
